Microbial biodiversity in arable soils is affected by agricultural practices
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Department of Biochemistry and Environmental Chemistry, The John Paul II Catholic University of Lublin, Konstantynów 1i, 20-708 Lublin, Poland
Faculty of Biology and Biotechnology, Department of Microbiology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1a, 10-719 Olsztyn, Poland
Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics PAS, Pawińskiego 5a, 02-206 Warsaw, Poland
Department of Microbial Biology, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland
Publication date: 2017-04-05
Int. Agrophys. 2017, 31(2): 259–271
The aim of the study was to examine the differences in microbial community structure as a result of agricultural practices. Sixteen samples of cultivated and the same number of non-cultivated soils were selected. Gel bands were identified using the GelCompar software to create the presence-absence matrix, where each band represented a bacterial operational taxonomic unit. The data were used for principal-component analysis and additionally, the Shannon-Weaver index of general diversity, Simpson index of dominance and Simpson index of diversity were calculated. Denaturing gradient gel electrophoresis profiles clearly indicated differentiation of tested samples into two clusters: cultivated and non-cultivated soils. Greater numbers of dominant operational taxonomic units (65) in non-cultivated soils were noted compared to cultivated soils (47 operational taxonomic units). This implies that there was a reduction of dominant bacterial operational taxonomic units by nearly 30% in cultivated soils. Simpson dominance index expressing the number of species weighted by their abundance amounted to 1.22 in cultivated soils, whereas a 3-fold higher value (3.38) was observed in non-cultivated soils. Land-use practices seemed to be a important factors affected on biodiversity, because more than soil type determined the clustering into groups.