3D-analysis of plant microstructures: advantages and limitations of synchrotron X-ray microtomography
U. Matsushima 1
W. Graf 2, 3
S. Zabler 4
I. Manke 5
M. Dawson 6
G. Choinka 5
A. Hilger 5
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Faculty of Agriculture, Iwate University, 3-18-8, Ueda, Morioka, Iwate, 020-8550, Japan
Department of Horticultural Engineering, Leibniz Institute for Agricultural Engineering Potsdam-Bornim, Max-Eyth-Allee 100, 14469 Potsdam, Germany
Kuratorium für Technik und Bauwesen in der Landwirtschaft, Bartningstraße 49, 64289 Darmstadt, Germany
Institute for Physics and Astronomy – Chair for X-ray microscopy, Julius-Maximillians Universitäty of Würzburg, Campus Hubland Nord, Josef-Martin-Weg 63, 97074 Würzburg, Germany
SF3, Helmholtz Center Berlin for Materials and Energy, Hahn-Meitner-Platz 1, 14109 Berlin, Germany
University of Salford, Newton Building 164, Salford, Greater Manchester, M5 4WT, UK
Int. Agrophys. 2013, 27(1): 23–30
Synchrotron X-ray computer microtomography was used to analyze the microstructure of rose peduncles. Samples from three rose cultivars, differing in anatomy, were scanned to study the relation between tissue structure and peduncles mechanical strength. Additionally, chlorophyll fluorescence imaging and conventional light microscopy was applied to quantify possible irradiation-induced damage to plant physiology and tissue structure. The spatial resolution of synchrotron X-ray computer microtomography was sufficiently high to investigate the complex tissues of intact rose peduncles without the necessity of any preparation. However, synchrotron X-radiation induces two different types of damage on irradiated tissues. First, within a few hours after first X-ray exposure, there is a direct physical destruction of cell walls. In addition, a slow and delayed destruction of chlorophyll and, consequently, of photosynthetic activity occurred within hours/days after the exposure. The results indicate that synchrotron X-ray computer microtomography is well suited for three-dimensional visualization of the microstructure of rose peduncles. However, in its current technique, synchrotron X-ray computer microtomography is not really non-destructive but induce tissue damage. Hence, this technique needs further optimization before it can be applied for time-series investigations of living plant materials.