Detection of the entrance of Lugol’s solution into the aleurone layer during germination
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Czech University of Life Sciences Prague, Faculty of Engineering, Department of Physics, Kamýcká 129, 165 00 Prague 6 - Suchdol, Czech Republic
Acceptance date: 2019-03-06
Publication date: 2019-07-19
Int. Agrophys. 2019, 33(3): 383-388
The movement of water into dry kernels is a critical step in germination, preharvest sprouting, and in the expression of dormancy. The soaking of kernels in Lugol’s solution allows for water uptake visualization by staining the cellular membranes and starch tissue. It is possible to recognize a stained aleurone layer during germination via image analysis (without cutting). A new method for the detection of Lugol’s solution in the aleurone layer of four wheat varieties is presented. The first entrance of the solution into the aleurone layer was detected. The rate at which the solution spread through the aleurone layer was also demonstrated. It was determined that the initial entrance time of the solution into the aleurone layer was different for the individual varieties (1.5-6 h) whereas the rate at which it spread in the aleurone layer was similar for the tested varieties and takes approximately 0.2-0.35 mm h-1. It was established that there is a close correlation between the solution speed in the aleurone layer and the mass increase during solution uptake for 3 out of 4 varieties.
Abenavoli M.R., Cacco G., Sorgonà A., Marabottini R., Paolacci A.R., Ciaffi M., and Badiani M., 2006. The inhibitory effects of coumarin on the germination of durum wheat (Triticum turgidum ssp. durum, CV. Simeto) seeds. J. Chem. Ecol., 32, 489-506. -005-9011-x.
ASABE Standards, 2006. S358.2 Moisture measurement-Forages. St. Joseph, Michigan, USA.
Bewley J.D., 1997. Seed germination and dormancy, Plant Cell, 9, 1055-1066.
Dell’Aquila A.D., 2006. Computerized seed imaging: a new tool to evaluate germination quality. Communications in Biometry Crop Sci., 1, 20-31.
Dell B., 1980. Structure and function of the strophiolar plug in seeds of Albizia lophantha. Am. J. Bot., 67, 556-563.
Dong K., Zhen S., Cheng Z., Cao H., Ge P., and Yan Y., 2015. Proteomic analysis reveals key proteins and phosphoproteins upon seed germination of wheat (Triticum aestivum L.). Front. Plant Sci., 6, 1 14.
Dunn O.J., 1964. Multiple comparisons using rank sums. Technometrics, 6, 241-252.
Gonzalez R.C. and Woods R.E., 2002. Digital Image Processing. Prentice Hall, New Jersey, USA.
Govender M., Chetty K., and Bulcock H., 2007. A review of hyperspectral remote sensing and its application in vegetation and water resource studies. Water SA, 33, 145-152.
Gruwel M.L.H., Chatson B., Yin X.S., and Abrams S., 2001. A magnetic resonance study of water uptake in whole barley kernels. Int. J. Food Sci. Technol., 36, 161-168.
Harb A.M., 2013. Reserve mobilization, total sugars and proteins in germinating seeds of durum wheat (Triticum durum Desf.) under water deficit after short period of imbibition. Jordan J. Biological Sci., 6, 67-72. 0000261.
Kikuchi K., Koizumi M., Ishida N., and Kano H., 2006. Water uptake by dry beans observed by micro-magnetic resonance. Imaging Annals of Botany, 98, 545-553.
King R.W., 1984. Water uptake in relation to pre-harvest sprouting damage in wheat: grain characteristics. Aust. J. Agr. Res., 35, 337 - 345.
Kornarzynski K., Pietruszewski S., and Lacek R., 2002. Measurement of the water absorption rate in wheat grain. Int. Agrophys., 16, 33-36.
Lancelot E., Bertrand D., Hanafi M., and Jaillais B., 2017. Near-infrared hyperspectral imaging for following imbibition of single wheat kernel sections. Vib. Spectrosc., 92, 46-53.
Lev J., Křepčík V., Prošek V., and Kumhála F., 2017. Capacitive throughput sensor for plant materials - Effects of frequency and moisture content. Computers Electronics Agric., 133, 22-29.
Lev J., and Blahovec J., 2017. Imbibition of wheat seeds: Application of image analysis. Int. Agrophys., 31, 475-481.
Lev J., and Blahovec J., 2018. Effect of I2/KI water solution to wheat seeds imbibition assessed by image analysis. Agronomy Research, 16, 492-499.
Manley M., du Toit G., and Geladi P., 2011. Tracking diffusion of conditioning water in single wheat kernels of different hardnesses by near infrared hyperspectral imaging. Analytica Chimica Acta, 686, 64-75. 1016/j.aca.2010.11.042.
McDonald M.B., Vertucci C.W., and Roos E.E., 1988. Seed coat regulation of soybean seed imbibition. Crop Sci., 28, 987-992.
Nakanishi T.M. and Matsubayashi M., 1997. Water imaging of seeds by neutron beam. Bioimages, 5, 45-48.
Rathjen J.R., Strounina E.V., and Mares D.J., 2009. Water movement into dormant and non-dormant wheat (Triticum aestivum L.) grains. J. Exp. Botany, 60, 1619-1631.
Resio A.C., Aguerre R.J., and Suarez C., 2006. Hydration kinetics of amaranth grain. Food Eng., 72, 247-253.
Ribeiro-Oliveira J.P. and Ranal M.A., 2017. Sample size and water dynamics on germinating diaspores: the first step for physiological and molecular studies on the germination process. Plant Biosyst., 152, 840-847.
Rokitta M., Zimmermann U., and Haase A., 1999. Fast NMR flow measurements in plants using FLASH imaging. J. Magn. Reson., 137, 29-32. 1998.1611.
Ünal H., Alpsoy H.C., and Ayhan A., 2013. Effect of the moisture content on the physical properties of bitter gourd seed. Int. Agrophys., 27, 455-461. -2013-0016.
Weitbrecht K., Müller K., and Leubner-Metzger G., 2011. First off the mark: early seed germination. J. Exp. Botany, 62, 3289-3309.
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