RESEARCH PAPER
ABA alleviated soybean seedling stress exposed to UV-C radiation
Liyan Yang 1  
,  
Ning Gao 1  
,  
Yashu Yang 1  
,  
Yurong Zhang 1  
,  
Jing Gao 1  
,  
Yi Sun 2  
 
 
More details
Hide details
1
Life Science Department, Shanxi Normal University, Linfen, China
2
Biotechnology Research Center, Shanxi Academy of Agricultural Sciences, Taiyuan, China
CORRESPONDING AUTHOR
Yi Sun   

sunyi692003@163.com
Publication date: 2020-01-16
Final revision date: 2019-09-14
Acceptance date: 2019-10-21
 
Int. Agrophys. 2020, 1(34): 115–121
KEYWORDS
TOPICS
ABSTRACT
In order to understand the roles of abscisis acid in soybean exposed to UV-C radiation, soybean seedlings were exposed to UV-C radiation and ABA was applied. The chlorophyll content, active oxygen metabolism and flavonoids content of soybean seedlings were investigated. The present study revealed that UV-C radiation significantly attenuated the chlorophyll content and suppressed PSII activity. It was observed that the contents of H2O2, malondialdehyde and O2- were markedly increased, and the activities of antioxidant enzymes such as superoxide dismutase, peroxidase and catalase were enhanced, so were the flavonoids content, whereas the seedling height and biomass were significantly reduced. ABA application improved the activities of superoxide dismutase, peroxidase, catalase and the contents of flavonoids, decreased the contents of H2O2, MDA and O2-, and also increased the chlorophyll content and enhanced PSII performance, thus enhancing biomass accumulation. The results suggested that ABA alleviated UV-C damage to soybean seedlings by reducing ROS generation and enhancing antioxidase activity. The isoflavones daidzin and genistein were significantly responsive to UV-C.
 
REFERENCES (21)
1.
Akito K., Kimitsune I., Takayuki K., Hideyuki M., and Kosaku T., 2015. Abscisic acid induces biosynthesis of bisbibenzyls and tolerance to UV-C in the liverwort Marchantia polymorpha. Phytochemistry, 117, 547-553. https://doi.org/10.1016/j.phyt....
 
2.
Amjad M., Akhtar J., and Yang AZ., 2014. Intergrating role of ethylene and ABA in tomato plants adaptation to salt stress. Sci. Hortic., 172, 109-116. https://doi.org/10.1016/j.scie....
 
3.
Asakawa Y., Ludwiczuk A., and Nagashima F., 2013. Phyto-chemical and biological studies of bryophytes. Phytochemistry, 91, 52-80. https://doi.org/10.1016/j.phyt....
 
4.
Bradford M.M., 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248-254. https://doi.org/10.1006/abio.1....
 
5.
Formica-Oliveira A.C., Díaz-López V., Artés F., and Artés-Hernández F., 2017. Use of postharvest uv-b and uv-c radiation treatments to revalorize broccoli byproducts and edible florets. Innovative Food Sci. Emerging Technol., 43, 77-83. https://doi.org/10.1016/j.ifse....
 
6.
Harrowven D.C. and Kostiuk S.L., 2012. Macrocyclic bisbibenzyl natural products and their chemical synthesis. Nat. Prod. Rep., 29, 223-242. https://doi.org/10.1039/c1np00....
 
7.
Hollosy F., 2002. Effects of ultraviolet radiation on plant cell. Micron., 33, 179-197. https://doi.org/10.1016/s0968-....
 
8.
Jasen M.A. and Bornman J.F., 2012. UV-B radiation: from generic stressor to specific regulator. Physiol Plant, 145, 501-504. https://doi.org/10.1111/j.1399....
 
9.
Lee H.S., 2000. Principles and Experimental Techniques of Plant Physiology and Biochemistry (in Chinese). Higher Education Press, Beijing, China.
 
10.
Li P., Cheng L., Gao H., Jiang C., and Peng, T., 2009. Heterogeneous behavior of PSII in soybean (glycine max) leaves with identical PSII photochemistry efficiency under different high temperature treatments. J. Plant Physiol., 166(15), 1607-1615. https://doi.org/10.1016/j.jplp....
 
11.
Ma M., Wang P., Yang R., and Gu Z.X., 2018. Effects of UV-B radiation on the isoflavone accumulation and physiological biochemical changes of soybean during germination Physiological-biochemical change of germinated soybean induced by UV-B. Food Chem., 250, 259-267. https://doi.org/10.1016/j.food....
 
12.
Mittova V., Tal M., Volokita M., and Guy M., 2002. Salt stress induces up-regulation of an efficient chloroplast antioxidant system in the salt-tolerant wild tomato species Lycopersicon pennellii but not in the cultivated species. Physiol. Plant, 115, 393-400. https://doi.org/10.1034/j.1399....
 
13.
Setlow R.B., 1974. The wavelengths in sunlight effective in producing skin cancer: a theoretical analysis. Proc. Nat. Acad. Sci., USA, 71, 3363-3366. https://doi.org/10.1073/pnas.7....
 
14.
Thiruvengadam M., Kim S.H., and Chung I.M., 2015. Exogenous phytohormones increase the accumulation of health-promoting metabolites, and influence the expression patterns of biosynthesis related genes and biological activity in Chinese cabbage (Brassica rapa spp. pekinensis). Sci. Hortic., 193, 136-146. https://doi.org/10.1016/j.scie....
 
15.
Tossi V., Cassia R., Bruzzone S., Zocchi E., and Lamattina L., 2012. ABA says NO to UV-B: a universal response? Trends Plant Sci., 17, 510-517. https://doi.org/10.1016/j.tpla....
 
16.
Urban L., Charles F., Miranda M.R.D., and Aarrouf J., 2016. Understanding the physiological effects of UV-C light and exploiting its agronomic potential before and after harvest. Plant. Physiol. Bioch., 105, 1-11. https://doi.org/10.1016/j.plap....
 
17.
Vicente A.R.,Pineda C., Lemoine L., Civello P.M., Martinez G.A., Chaves A.R., 2005. UV-C treatments reduce decay, retain quality and alleviate chilling injury in pepper. Postharvest Biol. Tec., 35, 69-78. https://doi.org/10.1016/j.post....
 
18.
Wu X., Guan W., Yan R., Lei J., Xu L., and Wang Z., 2016. Effects of UV-C on antioxidant activity, total phenolics and main phenolic compounds of the melanin biosynthesis pathway in different tissues of button mushroom. Post-harvest Biol. Technol., 118, 51-58. https://doi.org/10.1016/j.post....
 
19.
Yang R.C., Yang T., and Zhang H.J., 2014. Hormone profiling and transcription analysis reveal a major role of ABA in tomato salt tolerance. Plant Physiol. Bioch., 77, 23-24. https://doi.org/10.1016/j.plap....
 
20.
Ye B.X., and Zhu X.C., 2007. Basic experiments of biological science (in Chinese). Higher Education Press, Beijing, China.
 
21.
Zhou X., Peng J., Fan G., and Wu Y., 2005. Isolation and purification of flavonoid glycosides from Trollius lebebouri using high-speed counter-counter chromatography by stepwise increasing the flow-rate of the mobile phase. J. Chromatogr. A, 1092, 216-221. https://doi.org/10.1016/j.chro....
 
eISSN:2300-8725
ISSN:0236-8722