Assessment of the impact of spent mushroom substrate on biodiversity and activity of soil bacterial and fungal populations based on classical and modern soil condition indicators
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Department of Environmental Microbiology, Faculty of Agrobioengineering, University of Life Sciences in Lublin, Poland
Department of Herbology and Plant Cultivation Techniques, Faculty of Agrobioengineering, University of Life Sciences in Lublin, Poland
Institute of Plant Genetics, Breeding and Biotechnology, Faculty of Agrobioengineering, University of Life Sciences in Lublin, Poland
Final revision date: 2024-02-12
Acceptance date: 2024-02-16
Publication date: 2024-03-01
Corresponding author
Jolanta Joniec   

Department of Environmental Microbiology, Faculty of Agrobioengineering, University of Life Sciences in Lublin, Poland
Int. Agrophys. 2024, 38(2): 139-154
  • Microbial activity and biodiversity of soil treated by spent mushroom substrate was analysed
  • The waste had a positive effect on the development of microorganisms, but only in the first year
  • Fertilization with waste had a similar effect on soil microorganisms as manure
  • Results suggest using of both classical methods and methods based on the analysis of DNA from soil
In the present study, biological indicators were used to assess the impact of applying spent mushroom substrate and manure on the soil environment. The use of spent mushroom substrate had a varied effect on the microorganisms. Stimulation was recorded in the abundance of copiotrophic bacteria and fungi, but only in the first year of the study. In the case of cellulolytic bacteria, this effect was visible only in single plots. Similar observations were also noted regarding the relative DNA content (in relation to the control), which increased for both bacteria and fungi after applying spent mushroom substrate. In the soil fertilized with spent mushroom substrate, a decrease in DNA concentration was observed, but only in the first and second year. For enzymatic activity, the use of spent mushroom substrate alone proved to be more favorable, but this effect was again observed only in the first year of the study. The application of manure caused similar changes as observed with the use of spent mushroom substrate. These observations indicate a similar impact of spent mushroom substrate and manure on the parameters tested. The research presented suggests the use of both classical methods and methods based on the analysis of DNA extracted from soil to study the impact of spent mushroom substrate on the activity of soil microbial populations.
This work was financially supported by the University of Life Sciences in Lublin (RKM/S/21/2020).
The authors declare that there is no conflict of interest regarding the publication of this paper.
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