Comparative effect of silver nanoparticles on maize rhizoplane microbiome in initial phase of plants growth
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Department of Microbiology and Biomonitoring, University of Agriculture in Krakow, Mickiewicza 21, 31-120 Kraków, Poland
Department of Entomology, Phytopathology and Molecular Diagnostics, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 17, 10-720 Olsztyn, Poland
Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Niezapominajek 8, 30-239 Krakow, Poland
Final revision date: 2024-02-13
Acceptance date: 2024-02-21
Publication date: 2024-03-21
Corresponding author
Anna Gorczyca   

Department of Microbiology and Biomonitoring, University of Agriculture in Krakow, Mickiewicza 21, 31-120, Krakow, Poland
Int. Agrophys. 2024, 38(2): 155-164
  • Due to the numerous applications, silver nanoparticles will be released into the environment in increasing amounts.
  • Effect of silver nanoparticles on the soil microbiome differs from silver nitrate.
  • Effect of silver nanoparticles on the soil microbiome depends on the surface properties of the stabilizing layer.
  • Silver nanoparticle exposure alters the rhizosphere microbiome composition.
The aim of the experiments was to evaluate shifts in the prokaryotic and eukaryotic microbiome of maize rhizoplanes treated with five forms silver nanoparticles with different surface properties, produced by chemical reduction of silver(V) nitrate. Metagenomic studies were performed using appropriate procedures to create NGS libraries and sequences to species. All silver nanoparticles forms used moderately limited the growth of maize, without significantly affecting normalized difference vegetation indexes. Significant shifts in the taxa of the microbiome while preserving biodiversity were noted under the influence of silver nanoparticles, and the reaction of bacteria and eukaryotes was different. The eukaryotic microbiome, richer in the studied substrate, turned out to be more sensitive, showing greater qualitative and quantitative changes than the bacteriome. silver nanoparticles did not reduce the occurrence of mycorrhizal fungi, enriched the occurrence of Acidobacteriota and, with the exception of trisodium citrate reduction/sodium borohydride stabilization type, enriched the beneficial bacteria of Devosia. Within silver nanoparticles, distinct effects have been demonstrated for type with trisodium citrate reduction/sodium borohydride stabilization versus cysteamine reduction/trisodium citrate stabilization versus group: hydroxylamine hydrochloride reduction, tannic acid reduction and trisodium citrate reduction. The beneficial changes in maize rhizoplane microbiome can be attributed special to silver nanoparticles reduced using hydroxylamine hydrochloride.
This work was financially supported by the Project NCN MINIATURA-3 2019/03/X/NZ9/00567 (2019-2020).
The Authors declare they have no conflict of interest
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