Effectiveness of the use of urease inhibitors in agriculture: a review
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Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, Poland
Anna Siczek   

Department of Soil and Plant System, Institute of Agrophysics, Polish Academy of Sciences, Poland
Final revision date: 2021-06-30
Acceptance date: 2021-07-02
Publication date: 2021-08-02
Int. Agrophys. 2021, 35(2): 197–208
  • Urea is prone to ammonia volatilization what negatively affects the environment.
  • Urease inhibitors are the future of agricultural development.
  • Urea fertilizers with urease inhibitors effects on crop yields efficiency are revised.
  • The factors affecting ammonia volatilization from urea-based fertilizers are discussed.
  • The topic of natural urease inhibitors is presented.
Urea is one of the most popular fertilizers in the world. In 2018, the global production capacity of urea reached the level of 210 million metric tonnes. Due to the fact that the world population is growing steadily, the demand for food is increasing, and thus also the consumption of urea. The use of urea-based fertilizers has negative consequences in the form of ammonia volatilization. The solution to this problem may be the use of urease inhibitors. Currently, inhibitors of synthetic origin are available on the market, while the use of inhibitors of natural origin is still being under trial. The use of the most commonly used urease inhibitor – NBPT together with urea causes a reduction in ammonia emissions and has a beneficial effect on crop productivity. At the same time, the search for inhibitors of natural origin is underway, which, apart from reducing ammonia volatilization into the atmosphere, could have a positive effect on crop yields. In this paper, recent advances in this field are reviewed.
Ankri S. and Mirelman D., 1999. Antimicrobial properties of allicin from garlic. Microbes Infect., 1(2), 125-129.
Awllia J.J.A., Al-Ghamdi M., Huwait E., Javaid S., Atia-tul-Wahab, Rasheed S., and Choudhary M.I., 2016. Flavonoids as natural inhibitors of jack bean urease enzyme. Letters in Drug Design and Discovery, 13, 243-249.
Bernardi C.C., Mota E.P., Cardosa R.D., Monte M.B.M., and Oliveira P.P.A., 2014. Ammonia volatilization from soil, dry-matter yield, and nitrogen levels of Italian ryegrass. Communications in Soil Science and Plant Analysis, 45 (2), 153-162.
Cantarella H., Otto R., Soares J.R., and Silva A.G.B., 2018. Agronomic efficiency of NBPT as a urease inhibitor: A review. J. Advanced Res., 13, 19-27.
Chen D.L., Freney J.R., Mosier A.R., and Chalk P.M., 1994. Reducing denitrification loss with nitrification inhibitors following presowing applications of urea to a cotton field. Australian J. Exp. Agric., 34 (1), 75-83.
Chittora D., Meena M., Barupal T., Swapnil P., and Sharma K., 2020. Cyanobacteria as a source of biofertilizers for sustainable agriculture. Biochem. Biophys. Rep., 22, 100737.
Chunmei X., Liping C., Song C., Guang C., Danying W., and Xiufu Z., 2020. Rhizosphere aeration improves nitrogen transformation in soil, and nitrogen absorption and accumulation in rice plants. Rice Sci., 27 (2), 162-174.
Claussen W. and Lenz F., 1995. Effect of ammonium and nitrate on net photosynthesis, flower formation, growth and yield of eggplants (Solanum melongena L.). Plant Soil, 171, 267-274.
Clay D.E., Malzer G.L., and Anderson J.L., 1990. Ammonia volatilization from urea as influenced by soil temperature, soil water content, and nitrification and hydrolysis inhibitors. Soil Sci. Soc. Am. J., 54 (1), 263-266.
Das S.K., Ghosh G.K., and Avasthe R., 2020. Application of biochar in agriculture and environment, and its safety issues. Biomass Conversion Biorefinery.
Dawar K., Zaman M., Rowarth J.S., Blennerhassett J., and Turnbull M.H., 2011. Urease inhibitor reduces N losses and improves plant-bioavailability of urea applied in fine particle and granular forms under field conditions. Agric., Ecosys. Environ., 144, 41-50.
Dawar K., Zaman M., Rowarth J.S., and Turnbull M.H., 2012. Applying urea with urease inhibitor (N-(n-butyl) thiophosphoric triamide) in fine particle application improves nitrogen uptake in ryegrass (Lolium perenne L.). Soil Sci. Plant Nutr., 58 (3), 309-318.
Dewi F.C., Putra E.T.S., and Wulandari C., 2018. The effect of urease inhibitors coated urea on the growth, physiological activities and yield of maize (Zea mays L.) in inceptisol Jogonalan, Klaten. Ilmu Pertanian. Agric. Sci., 3 (3), 160-165.
Ding Y., Liu Y., Liu S., Huang X., Li Z., Tan X., Zeng G., and Zhou L., 2017. Potential benefits of biochar in agricultural soils: a review. Pedosphere, 27 (4), 645-661.
Dong D., Kou Y., Yang W., Chen G., and Xu H., 2018. Effects of urease and nitrification inhibitors on nitrous oxide emissions and nitrifying/denitrifying microbial communities in a rainfed maize soil: A 6-year field observation. Soil Till. Res., 180, 82-90.
Drury C.F., Yang X., Reynolds W.D., Calder W., Oloya T.O., and Woodley A.L., 2017. Combining urease and nitrification inhibitors with incorporation reduces ammonia and nitrous oxide emissions and increases corn yields. J. Environ. Quality, 46, 939-949.
Espindula M.C., Rocha V.S., Souza M.A., Campanharo M., and Sousa Paula G., 2013. Rates of urea with or without urease inhibitor for topdressing wheat. Chilean J. Agric. Res., 73 (2), 160-167.
Fenn L.B. and Hossner L.R., 1985. Ammonia volatilization from ammonium or ammonium-forming nitrogen fertilizers. Advances Soil Sci., 1, 123-169.
Ferguson R.B., Kissel D.E., Koelliker J.K., and Basel E., 1984. Ammonia volatilization form surface-applied urea: effect of hydrogen ion buffering capacity. Soil Sci. Soc. Am. J., 48 (3), 578-582.
Fernando V. and Roberts G.R., 1976. The partial inhibition of soil urease by naturally occurring polyphenols. Plant Soil, 44, 81-86.
Follet R., 2008. Chapter 2. Transformation and Transport Processes. Nitrogen in the Environment: Sources, Problems, and Management.
Fu Q., Abadie M., Blaud A. et al., 2020. Effects of urease and nitrification inhibitors on soil N, nitrifier abundance and activity in a sandy loam soil. Biol. Fertil. Soils, 56, 185-194.
Gil M.V., Carballo M.T., and Calvo L.F., 2008. Fertilization of maize with compost from cattle manure supplemented with additional mineral nutrients. Waste Manag., 28 (8), 1432-1440.
Gilbert P.M., Harrison J., Heil C.A., and Seitzinger S., 2006. Escalating worldwide use of urea – a global change contributing to coastal eutrophication. Biogeochemistry, 77(3), 441-463.
Grant C.A. and Bailey L.D., 1999. Effect of seed-placed urea fertilizer and N-(n-butyl) thiophosphoric triamide (NBPT) on emergence and grain yield of barley. Canadian J. Plant Sci., 79 (4), 491-496.
Guardia G., Sanz-Cobena A., Sanchez-Martin L., Fuertes-Mendizabal T., Gonzalez-Murua C., Alvares J.M., Chadwick D., and Vallejo A., 2018. Urea-based fertilization strategies to reduce yield-scaled N oxides and enhance bread-making quality in a rainfed Mediterranean wheat crop. Agric. Ecosys. Environ., 265, 1, 421-431.
He Z.L., Alva A.K., Calvert D.V., and Banks D.J., 1999. Ammonia volatilization from different fertilizer sources and effects of temperature and soil pH. Soil Sci., 164(10), 750-758.
Hube S., Alfaro M.A., Scheer C., Brunk C., Ramirez L., Rowlings D., and Grace P., 2017. Effect of nitrification and urease inhibitors on nitrous oxide and methane emissions from an oat crop in a volcanic ash soil. Agric. Ecosys. Environ., 238, 46-54.
Jabri E., Carr M.B., Hausinger R., and Karplus P., 1995. The crystal structure of urease form Klebsiella aerogenes. Science (New York, N.Y.), 268, 998-1004.
Kafarski P. and Talma M., 2018. Recent advances in design of new urease inhibitors: A review. J. Advanced Res., 13,101-112.
Kalembasa S., Szukała J., Faligowska A., Kalembasa D., Symanowicz B., Becher M., and Gebus-Czupyt B., 2020. Quantification of biologically fixed nitrogen by white lupin (Lupins albus L.) and its subsequent uptake by winter wheat using the 15N isotope dilution method. Agronomy, 10, 1392;
Kappaun K., Piovesan A.R., Carlini C.R., and Ligabue-Braun R., 2018. Ureases: Historical aspects, catalytic, and non-catalytic properties – A review. J. Advanc. Res., 13, 3-17.
Kawakami E.M., Oosterhuis D.M., Snider J.L., and Mozaffari M., 2012. Physiological and yield responses of field-grown cotton to application of urea with the urease inhibitor NBPT and the nitrification inhibitor DCD. Eur. J. Agron., 43, 147-154.
Kiss S. and Simihặian M., 2002. Improving efficiency of urea fertilizers by inhibition of soil urease activity. Kluwer Academic Publishers; Doordrech.
Kissel D.E. and Cabrera M.L., 2005. Ammonia. References Module in Earth Systems and Environmental Sciences. Encyclopedia Soils Environ., 56-64.
Klimczyk M., Siczek A., and Schimmelpfennig L., 2021. Improving the efficiency of urea-based fertilization leading to reduction in ammonia emission. Science of the Total Environment, 771.
Lasisi A.A., Akinremi O.A., and Kumaragamage D., 2019. Efficacy of a new N-(n-butyl) thiophosphoric triamide formulation in reducing ammonia volatilization from urea-based fertilizers. Can. J. Soil Sci., 99, 395-405.
Li Q., Cui X., Liu X., et al., 2017. A new urease-inhibiting formulation decreases ammonia volatilization and improves maize nitrogen utilization in North China Plain. Sci. Rep., 7.
Li Q., Yang A., Wang Z., Roelcke M., Chen X., Zhang F., Pasda G., Zerulla W., Wissemeier A.H., and Liu X., 2015. Effect of a new urease inhibitor on ammonia volatilization and nitrogen utilization in wheat in north and northwest China. Field Crops Res., 175, 96-105.
Li X.G., Jia B., Lv J., Ma Q., Kuzyakov Y., and Li F., 2017. Nitrogen fertilization decreases the decomposition of soil organic matter and plant residues in planted soils. Soil Biology Biochem., 112, 47-55.
Liu X., Zhang M., Li Z., Zhang C., Wan C., Zhang Y., and Lee D.J., 2019. Inhibition of urease activity by humic acid extracted from sludge fermentation liquid. Bioresource Technol., 290.
Luchibia A.O., Suter H., Hu H., Lam S.K., and He J.-Z., 2020. Responses of ureolytic and nitrifying microbes to urease and nitrification inhibitors in selected agricultural soils in Victoria, Australia. J. Soils Sediments, 20, 1309-1322.
Marchesan E., Grohs M., Walter M., da Silva L.S., and Formentini T.C., 2013. Agronomic performance of rice to the use of urease inhibitor in two cropping systems. Revista Ciência Agronômica, 44 (3).
Martins M.R., Sant’Anna S.A.C., Zaman M., Santos R.C., Monteiro R.C., Alves B.J.R., Jantalia C.P., Boddey R.M., and Urquiaga S., 2017. Strategies for the use of urease and nitrification inhibitors with urea: Impact on N2O and NH3 emissions, fertilizer-15N recovery and maize yield in a tropical soil. Agric. Ecosys. Environ., 247, 54-62.
Mathialagan R., Mansor N., Al-Khateeb B., Mohamad M.H., and Shamsuddin M.R., 2017. Evaluation of Allicin as Soil Urease Inhibitor. Procedia Eng., 184, 449-459.
Modolo L.V., da-Silva C.J., Brandão D.S., and Chaves I.S., 2018. A minireview on what we have learned about urease inhibitors of agricultural interest since mid-2000s. J. Advan. Res., 13, 29-37.
Mulyani N.S., Suryadi M.E., Dwiningsih S., and Haryanto H., 2001. Nitrogen dynamics on rice field soils. J. Tanah dan Iklim, 19, 14-25.
Ni K., Kage H., and Pacholski A., 2018. Effects of novel nitrification and urease inhibitors (DCD/TZ and 2-NPT) on N2O emissions from surface applied urea: An incubation study. Atmosp. Environ., 75-82.
Papangkorn J., Isaraphan C., Phinhongthong S., Opaprakasit M., and Opaprakasit P., 2008. Controlled-release material for urea fertilizer from polylactic acid. Advan. Materials Res., 55-57, 897-900.
Pastene E., Troncoso M., Figueroa G., Alarcon J., and Hernan S., 2009. Association between polymerization degree of apple peel polyphenols and inhibition of Helicobacter pylori urease. J. Agric. Food Chem., 57(2), 416-424.
Peiris C., Gunatilake S.R., Wewalwela J.J., and Vithanage M., 2019. Chapter 11 - Biochar for Sustainable Agriculture: Nutrient Dynamics, Soil Enzymes, and Crop Growth. Biochar from Biomass and Waste, Fundamentals and Applications, 211-224.
Pelster D.E., Chantigny M.H., Angers D.A., Bertrand N., MacDonald J.D., and Rochette P., 2018. Can soil clay content predict ammonia volatilization losses from subsurface-banded urea in eastern Canadian soils? Canadian J. Soil Sci., 98(3): 556-565.
Qi X., Wu W., Shah F., Peng S., Huang J., Cui K., Liu H., and Nie L., 2012. Ammonia volatilization from urea-application influenced germination and early seedling growth of dry direct-seeded rice. The Scientific World J.,
Reeza A., Ahmed O., Majid N., and Jalloh M.B., 2009. Reducing ammonia loss from urea by mixing with humic and fulvic acids isolated from coal. Am. J. Environ. Sci., 5(3), 420-426.
Robertson, G.P. and Groffman P.M., 2015. Nitrogen transformations. Soil microbiology, ecology and biochemistry. 421-446. Academic Press, Burlington, Massachusetts, USA.
Sanz-Cobena A., Abalos D., Meijide A. Sanchez-Martin L., and Vallejo A., 2013. Soil moisture determines the effectiveness of two urease inhibitors to decrease N2O emission. Mitigation Adaptation Strategies for Global Change, 21, 1131-1144.
Shabana S., Kawai A., Kai K., Akiyama K., and Hayashi H., 2010. Inhibitory activity against urease of quercetin glycosides isolated from Allium cepa and Psidium guajava. Biosci. Biotechnol. Biochem., 74 (4), 878-880.
Shamsuddin R., Osumanu H.A., and Nik M.A.M., 2009. Controlling ammonia volatilization by mixing urea with humic acid, fulvic acid, triple superphosphate and muriate of potash. Am. J. Environ. Sci., 5(5), 605-609.
Shan L., He Y., Chen J., Huang Q., and Wang H., 2015. Ammonia volatilization from a Chinese cabbage field under different nitrogen treatments in the Taihu Lake Basin, China. J. Environ. Sci., 38, 14-23.
Sidi N., Aris A.Z., Talib S.N., Johan S., Yusoff T.S.T.M., and Ismail M.Z., 2015. Influential factors on the cation exchange capacity in sediment of Merambong Shoal, Johor. Procedia Environ. Sci., 30, 186-189.
Silva A.G.B., Sequeira C.H., Sermarini R.A., and Otto R., 2017. Ureaseinhibitor NBPT on ammonia volatilization and crop productivity: A meta‐analysis. Agronomy J., 109(1), 1-13.
Sommer S.G., Schjoerring J.K., and Denmead O., 2004. Ammonia emission from mineral fertilizers and fertilized crops. Advances Agronomy, 82, 557-622.
Sullivan D.M. and Havlin J.L., 1992. Soil and environmental effects on urease inhibition by ammonium thiosulfate. Soil Sci. Soc. Am. J., 56 (3), 950-956.
Sun X., Zhong T., Zhang L., Zhang K., and Wu W., 2019. Reducing ammonia volatilization from paddy field with rice straw derived biochar. Sci. Total Environ., 660, 512-518.
Svane S., Siguardson J.J., Finkenwirth F., Eitinger T., and Karring H., 2020. Inhibition of urease activity by different compounds provides insight into the modulation and association of bacterial nickel import and ureolysis. Sci. Report, 10, 8503.
Trenkel M.E., 2010. Slow- and controlled-release and stabilized fertilizers: An Option for Enhancing Nutrient Use Efficiency in Agriculture. Int. Fertilizer Industry Association: Paris, France, 61-67, 18-20.
Veresoglou S.D. and Menexes G., 2010. Impact of inoculation with Azospirillum spp. on growth properties and seed yield of wheat: a meta-analysis of studies in the ISI Web of Science from 1981 to 2008. Plant Soil, 337:469-480.
Wallace A.J., Armstrong R.D., Grace P.R., Scheer C., and Partington D.L., 2020. Nitrogen use efficiency of 15N urea applied to wheat based on fertiliser timing and use of inhibitors. Nutrient Cycling Agroecos., 116, 41-56.
Wang H., Köbke S., and Dittert K., 2020. Use of urease and nitrification inhibitors to reduce gaseous nitrogen emissions from fertilizers containing ammonium nitrate and urea. Global Ecol. Conserv., 22.
Wang X., Zhang L., Zou J., and Liu S., 2015. Optimizing net greenhouse gas balance of a bioenergy cropping system in southeast China with urease and nitrification inhibitors. Ecol. Eng., 83, 191-198.
Watson C.J., Miller H., Poland P., Kilpatrick D.J., Allen M.D.B., Garrett M.K., and Christianson C.B., 1994. Soil properties and the ability of the urease inhibitor N-(n-BUTYL) thiophosphoric triamide (nBTPT) to reduce ammonia volatilization from surface-applied urea. Soil Biol. Biochem., 26 (9), 1165-1171.
Wesołowska M., Rymarczyk J., Góra R., Baranowski P., Sławiński C., Klimczyk M., Supryn G., and Schimmelpfennig L., 2021. New slow-release fertilizers – economic, legal and practical aspects: a Review. Int. Agrophys., 35, 11-24.
Zaman M., Saggar S., Blennerhassett J.D., and Singh J., 2009. Effect of urease and nitrification inhibitors on N transformation, gaseous emissions of ammonia and nitrous oxide, pasture yield and N uptake in grazed pasture system. Soil Biol. Biochem., 41, 1270-1280.
Zhang S., Yuan L., Li W., Lin Z., Li Y., Hu S., and Zhao B., 2019. Effects of urea enhanced with different weathered coal-derived humic acid components on maize yield and fate of fertilizer nitrogen. J. Integrative Agric., 18(3), 656-666.
Zhao T.X.Z., Arshad M., Li N., Zare E., and Triantafilis J., 2020. Determination of the optimal mathematical model, sample size, digital data and transect spacing to map CEC (Cation exchange capacity) in a sugarcane field. Computers Electronics Agric., 173.
Zhenghu D. and Honglang X., 2000. Effects of soil properties on ammonia volatilization. Soil Sci. Plant Nutr., 46(4), 845-852.
Zhu H., Yang J., Yao R., Wang X., Xie W., Zhu W., Liu X., Cao Y., and Tao J., 2020. Interactive effects of soil amendments (biochar and gypsum) and salinity on ammonia volatilization in coastal saline soil. Catena, 190.