Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, Poland
Final revision date: 2026-08-13
Acceptance date: 2026-09-04
Corresponding author
Artur Zdunek
Microstructure and Mechanics of Biomaterials, Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290, Lublin, Poland
This mini-review focuses on the recent usage of pectin for 3D bioprinting in biomedical and food industry applications by highlighting the design and functional optimisation of bioinks. It discusses the structural features of pectin, effects of physicochemical factors influencing gelation, designing of pectin-based bioinks, and applications of pectin based bioinks across various fields. Furthermore, it investigates the recent advancements in this field by tuning the rheological properties by crosslinking and blending with other polymers which eventually improvise the printing and stability of the printed constructs. It also discusses, current limitations including reproducibility and strategy for standardised formulation along with future directions in this field.
FUNDING
This work was supported by the National Science Centre, Poland (grant number 2023/49/B/NZ9/02979).
CONFLICT OF INTEREST
The authors declare that they have no conflict of interest.
AUTHORS' CONTRIBUTIONS
APV – writing, editing, graphics; JC – writing, editing; SB – reviewing; AZ – writing, reviewing, supervision, editing. All authors have read and agreed to the published version of the manuscript.
REFERENCES(69)
1.
Abraham, S., Furtado, S., Krishnamurthy, S., Awad, O., Prajapati, B., 2025. Pectin based three dimensional printing in wound healing: Innovations, challenges, and future prospects: A review. Carbohydrate Polymer Technologies Applications 11, 100976. https://doi.org/10.1016/j.carp....
Altun, F., Bayar, A., Hamzat, A.K., Asmatulu, R., Ali, Z., Asmatulu, E., 2025. AI-driven innovations in 3D printing: optimization, automation, and intelligent control. J. Manufacturing Materials Proce. 9, 329. https://doi.org/10.3390/jmmp91....
Andriotis, E.G., Eleftheriadis, G.K., Karavasili, C., Fatouros, D.G., 2020a. Development of bio-active patches based on pectin for the treatment of ulcers and wounds using 3D-bioprinting technology. Pharmaceutics 12(1), 56. https://doi.org/10.3390/pharma....
Andriotis, E.G., Monou, P.K., Louka, A., Papaefstathiou, E., Eleftheriadis, G.K., Fatouros, D.G., 2020b. Development of food grade 3D printable ink based on pectin containing cannabidiol/cyclodextrin inclusion complexes. Drug Development Industrial Pharmacy 46(10), 1569-1577. https://doi.org/10.1080/036390....
Bugday, Y., Alicke, A., Anderson, P., van der Sman R., 2026. Understanding the large deformation response of paste-like 3D food printing inks. Applied Rheology 35(1), 20250042. https://doi.org/10.1515/arh-20....
Bulone, D., Giacomazza, D., Manno, M., Martorana, V., San Bagio, P.L., 2015. Sucrose pectin interaction from solution to gels. Food Hydrocolloids: Characteristics, Properties and Structures, 6, 225-241.
Cao, L., Lu, W., Mata, A., Nishinari, K., Fang, Y., 2020. Egg-box model-based gelation of alginate and pectin: A review. Carbohydrate Polymers 242, 116389. https://doi.org/10.1016/j.carb....
Chimene, D., Lennox, K.K., Kaunas, R.R., Gaharwar, A.K., 2016. Advanced bioinks for 3D printing: A materials science perspective. Annals of Biomedical Eng. 44(6), 2090-2102. https://doi.org/10.1007/s10439....
Derossi, A., Spence, C., Corradini, M.G., Jekle, M., Fahmy, A. R., Caporizzi, R., et al., 2024. Personalized, digitally designed 3D printed food towards the reshaping of food manufacturing and consumption. Npj Science Food 8(1), 1-10. https://doi.org/10.1038/s41538....
Erandi, N., Fan, M., Choi, Y., Kim, E., 2023. Food hydrocolloids effect of pectin on printability and textural properties of potato starch 3D food printing gel during cold storage. Food Hydrocolloids, 137, 108362. https://doi.org/10.1016/j.food....
Fiedot, M., Lesiak, D., Podstawczyk, D., Ujčić, A., Kryszak B., Kockova, O., et al., 2025. Citrus or apple inks for the 3D printing – the influence of the low methylated pectin structure and the standardized sugar type on their printability. Int. J. Biol. Macromol. 330 (Pt 2): 147672. https://doi.org/10.1016/j.ijbi....
Fu, Z., Angeline, V., Sun, W., 2021. Evaluation of printing parameters on 3D extrusion printing of pluronic hydrogels and machine learning guided parameter recommendation. Int. J. Bioprinting 7(4), 179-189. https://doi.org/10.18063/IJB.V....
Gao, Q., Kim, B.S., Gao, G., 2021. Advanced strategies for 3D bioprinting of tissue and organs analogs using alginate hydrogel bioinks. Marine Drugs 19(12), 708. https://doi.org/10.3390/md1912....
Gawkowska, D., Ciesla, J., Zdunek, A., Cybulska, J., 2019. The effect of concentration on the cross-linking and gelling of sodium carbonate-soluble apple pectins. Molecules 24(8), 1635. https://doi.org/10.3390/molecu....
Gawkowska, D., Cybulska, J., Zdunek, A., 2018. Structure-related gelling of pectins and linking with other natural compounds: A review. Polymers 10(7), 762. https://doi.org/10.3390/polym1....
Gu, M., Gu, H., Raghavan, V., Wang, J., 2024. Ultrasound treatment improved the physicochemical properties of pea protein with pectin ink used for 3D printing. Future Foods 9, 100377. https://doi.org/10.1016/j.fufo....
Gungor-Ozkerim, P.S., Inci, I., Zhang, Y.S., Khademhosseini, A., Dokmeci, M.R., 2018. Bioinks for 3D bioprinting: An overview. Biomaterials Science 6(5), 915-946. https://doi.org/10.1039/c7bm00....
Hidaka, M., Kojima, M., Nakahata, M., Sakai, S., 2021. Visible light-curable chitosan ink for extrusion-based and vat polymerization-based 3D bioprintings. Polymers 13(9), 1382. https://doi.org/10.3390/polym1....
Huang, J.H.R., Wu, C.Y., Chan, H.M., Ciou, J.Y., 2022. Printing parameters of sugar/pectin jelly candy and application by using a decision tree in a hot-extrusion 3D printing system. Sustainability 14(18), 11618. https://doi.org/10.3390/su1418....
Iftekar, S.F., Aabid, A., Amir, A., Baig, M., 2023. Advancements and limitations in 3D printing materials and technologies: A Critical Review. Polymers 15(11), 2519. https://doi.org/10.3390/polym1....
Kameshwar, A.K.S., Qin, W., 2018. Structural and functional properties of pectin and lignin-carbohydrate complexes de-esterases: A review. Bioresources Bioprocessing 5(1), 1-16. https://doi.org/10.1186/s40643....
Keerthy, A., Dhanaselvam, R., Santhoshkumar, Ravikrishnan, V., Arthur, J., 2025. Improving the printability of watermelon rind using pectin: 3D printing optimization for the development of gummies. Food Biomacromolecules 2, 143-156. https://doi.org/10.1002/fob2.7....
Kim, Y.H., Priyadarshi, R., Kim, J.W., Kim, J., Alekseev, D.G., Rhim, J.W., 2022. 3D-printed pectin/carboxymethyl cellulose/ZnO bio-inks: Comparative analysis with the solution casting method. Polymers 14(21), 4711. https://doi.org/10.3390/polym1....
Lapomarda, A., Pulidori, E., Cerqueni, G., Chiesa, I., De Blasi, M., Geven, M.A., et al., 2021. Pectin as rheology modifier of a gelatin-based biomaterial ink. Materials 14(11), 1-16. https://doi.org/10.3390/ma1411....
Lootens, D., Durand, D., Nicolai, T., Boulenguer, P., Langendorff, V., 2003. Influence of pH, Ca concentration, temperature and amidation on the gelation of low methoxyl pectin. Food Hydrocolloids 17, 237-244. https://doi.org/10.1016/S0268-....
Ma, T., Lv, L., Ouyang, C., Hu, X., Liao, X., Song, Y., et al., 2021. Rheological behavior and particle alignment of cellulose nanocrystal and its composite hydrogels during 3D printing. Carbohydrate Polymers 253, 117217. https://doi.org/10.1016/j.carb....
Merli, M., Sardelli, L., Baranzini, N., Grimaldi, A., Jacchetti, E., Raimondi, M.T., et al., 2022. Pectin-based bioinks for 3D models of neural tissue produced by a pH-controlled kinetics. Frontiers in Bioengineering and Biotechnology 10, 1-22. https://doi.org/10.3389/fbioe.....
Mierczyńska, J., Cybulska, J., Zdunek, A., 2017. Rheological and chemical properties of pectin enriched fractions from different sources extracted with citric acid. Carbohydrate Polymers 156, 443-451. https://doi.org/10.1016/j.carb....
Morris, G.A., Castile, J., Smith, A., Adams, G.G., Harding, S.E., 2010. The effect of different storage temperatures on the physical properties of pectin solutions and gels. Polymer Degradation Stability 95(12), 2670-2673. https://doi.org/10.1016/j.poly....
Ngouémazong, D.E., Jolie, R.P., Cardinaels, R., Fraeye, I., Loey, A., Van, Moldenaers, P., et al., 2012. Stiffness of Ca2+-pectin gels: combined effects of degree and pattern of methylesterification for various Ca2+ concentrations. Carbohydrate Research 348, 69-76. https://doi.org/10.1016/j.carr....
Pasarin, D., Ghizdareanu, A., Teodorescu, F., Rovinaru, C., Banu, A., 2023. Characterization of pectin oligosaccharides obtained from citrus peel pectin. Fermentation 9(3), 312 1-13. https://doi.org/10.3390/fermen....
Passamai, V.E., Katz, S., Rodenak-Kladniew, B., Alvarez, V., Castro, G.R., 2023. Pectin-based inks development for 3D bioprinting of scaffolds. J. Polymer Res. 30, 35. https://doi.org/10.1007/s10965....
Patitucci, F., Motta, M.F., Dattilo, M., Malivindi, R., Leonetti, A. E., Pezzi, G., et al., 2024. 3D-printed alginate/pectin-based patches loaded with olive leaf extracts for wound healing applications: development, characterization and in vitro evaluation of biological properties. Pharmaceutics 16(1), 99. https://doi.org/10.3390/pharma....
Pereira, R.F., Sousa, A., Barrias, C.C., Bártolo, P.J., Granja, P.L., 2018. A single-component hydrogel bioink for bioprinting of bioengineered 3D constructs for dermal tissue engineering. Materials Horizons 5(6), 1100-1111. https://doi.org/10.1039/c8mh00....
Rubio-Martin del Campo, K.N., Rivas-Gastelum, M.F., Garcia-Amezquita, L.E., Sepulveda-Villegas, M., López-Mena, E.R., Mejía-Méndez, J.L., et al., 2025. From nature to science: A review of the applications of pectin-based hydrogels. Macromolecules 5, 58. https://doi.org/10.3390/macrom....
Saha D., Padhiary M., Hoque A., Prasad G., 2025. 3D printing technology for valorization of food processing wastes and byproducts: A systematic review. Waste Management Bull. 3, 100192. https://doi.org/10.1016/j.wmb.....
Shahrubudin, N., Lee, T.C., Ramlan, R., 2019. An overview on 3D printing technology: Technological, materials, and applications. Procedia Manufacturing 35, 1286-1296. https://doi.org/10.1016/j.prom....
Teixeira, M.C., Lameirinhas, N.S., Carvalho, J.P.F., Luís, J., Oliveira, H., Oliveira, J.M., et al., 2024. Biobased hydrogel bioinks of pectin, nanocellulose and lysozyme nanofibrils for the bioprinting of A375 melanoma cell-laden 3D in vitro platforms. Int. J. Biological Macromolecules 282(5), 136958. https://doi.org/10.1016/j.ijbi....
Vancauwenberghe, V., Baiye, V., Mbong, M., Vanstreels, E., Lammertyn, J., Nicolai, B., 2018a. 3D printing of plant tissue for innovative food manufacturing: Encapsulation of alive plant cells into pectin based bio-ink. J. Food Eng. 263, 454-464. https://doi.org/10.1016/j.jfoo....
Vancauwenberghe, V., Verboven, P., Lammertyn, J., Nicolaï, B., 2018b. Development of a coaxial extrusion deposition for 3D printing of customizable pectin-based food simulant. J. Food Eng. 225, 42-52. https://doi.org/10.1016/j.jfoo....
Wang, S., Tohti, M., Zhang, J., Li, J., Li, D., 2023. Acylhydrazone-derived whole pectin-based hydrogel as an injectable drug delivery system. Int. J. Biological Macromolecules 251, 126276. https://doi.org/10.1016/j.ijbi....
Yang, X., Yuan, K., Descallar, F.B.A., Li, A., Yang, X., Yang, H., 2022. Gelation behaviors of some special plant-sourced pectins: A review inspired by examples from traditional gel foods in China. Trends Food Sci Technol. 126, 26-40. https://doi.org/10.1016/j.tifs....
Zdunek, A., Pieczywek, P.M., Cybulska, J., 2021. The primary, secondary, and structures of higher levels of pectin polysaccharides. Comprehensive Reviews in Food Sci. Food Safety 20(1), 1101-1117. https://doi.org/10.1111/1541-4....
Zhu, W., Iskandar, M.M., Baeghbali, V., Kubow, S., 2023. Three-Dimensional printing of foods: A Critical Review of the Present State in Healthcare Applications, and Potential Risks and Benefits. Foods 12(17). https://doi.org/10.3390/foods1....
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.