Effect of mineral and organic additions on soil microbial composition
More details
Hide details
Department of Microbiology and Biomonitoring, University of Agriculture in Kraków, al. Mickiewicza 24/28, 30-059 Kraków, Poland
Department of Mineralogy, Petrography and Geochemistry, AGH University of Science and Technology, al. Mickiewicza 30, 30-059 Kraków, Poland
Department of Environmentalistics and Natural Resources, Mendel University in Brno, Zemědělská 1/1665, 613 00 Brno, Czech Republic
Department of Agricultural and Environmental Chemistry, University of Agriculture in Kraków, Mickiewicza 21, 31-120 Kraków, Poland
Katarzyna Wolny-Koładka   

Department of Microbiology and Biomonitoring, University of Agriculture in Krakow, Poland
Final revision date: 2022-04-01
Acceptance date: 2022-04-06
Publication date: 2022-05-25
Int. Agrophys. 2022, 36(2): 131–138
  • The microbiocenotic composition of the soil.
  • Innovative mineral-organic soil fertilization.
  • Cultivation of wheat and rape.
The aim of the study was to evaluate the effect of different mineral-organic mixtures on changes in soil microbial composition and chemical properties. The design of the pot experiment included 6 treatments: soil without fertilization – C, soil fertilized with mineral NPK fertilizers – MF, soil with NPK + 3 or 6% lignite and 3% zeolite-vermiculite composite (MF+CW3%, MF+CW6%), soil with NPK + 3 or 6% leonardite and 3% zeolite-vermiculite composite (MF+CL3%, MF+CL6%). The test plants were spring oilseed rape and spring wheat. The highest number of microorganisms was observed: for oilseed rape – in the soil of the MF+CW3% and MF+CW6% treatments, and for wheat – in the soil of the MF+CL3% and MF+CL6% treatments. The maximum percentage increase in the number of analysed microorganisms, for spring rape and spring wheat, respectively, was: bacteria 190% (MF+CW3%) and 1198% (MF+CL3%), mould fungi 221% (MF+CW3%) and 1601% (MF+CL3%), Azotobacter spp. 248% (MF+CW6%) and 251% (MF+CL3%), actinomycetes 116% (MF+CW3%) and 251% (MF+CL3%). The beneficial effect of the applied mineral-organic mixtures on soil biological activity is closely related to the effect of these materials on soil chemical properties, such as pH or electrical conductivity, which was confirmed by the calculated correlation coefficients.
This work was supported by the project “Fly ashes as precursors of functionalized materials for applications in environmental engineering, civil engineering and agriculture” – the project is carried out within the TEAM-NET programme of the Foundation for Polish Science POIR.04.04.00-00-14E6/18-00. (2020-2023).
The authors declare that they have no conflict of interest
Aciego Pietri J.C. and Brookes P.C., 2008. Relationships between soil pH and microbial properties in a UK arable soil. Soil Biol. Biochem., 40(7), 1856-1861,
Akinremi O.O., Janzen H.H., Lemke R.L., and Larney F.J., 2000. Response of canola, wheat and green beans to leonardite additions. Can. J. Soil Sci., 80(3), 437-443,
Alvarez A., Catalanos A., and Amorosom J., 2012. Heavy metal resistant strains are widespread along Streptomyces phylogeny. Mol. Phylogenet. Evol., 66, 1083-1088,
Atlas R.M. and Parks L.C., 1997. Handbook of Microbiological Media. CRC Press, Boca Raton, FL, USA.
Beheshti M., Etesami H., and Alikhani H.A., 2018. Effect of different biochars amendment on soil biological indicators in a calcareous soil. Environ. Sci. Pollution Res., 1-10,
Bereza-Boruta B., 2002. Selected enzymatic activities of actinomycetes of Streptomyces genus isolated from potato plantation (in Polish). Agricultura, 1, 27-36.
Burns R.G., De Forest J.L., Marxsen J., Sinsabaugh R.L., Stromberger M.E., Wallenstein M.D., Weintraub M., and Zoppini A., 2013. Soil enzymes in a changing environment: Current knowledge and future directions. Soil Biol. Biochem., 58, 216-234,
Dębska B., Maciejewska A., and Kwiatkowska J., 2002. The effect of fertilization with brown coal on Haplic Luvisol humic acids. Rostl. Vyroba, 48(1), 33-39,
Doni S., Gispert M., Peruzzi E., Macci C., Mattii G.B., Manzi D., Masini C.M., and Grazia M., 2020. Impact of natural zeolite on chemical and biochemical properties of vineyard soils. Soil Use Manag., 0-2,
Eslami M., Khorassani R., Fotovat A., and Halajnia A., 2020. NH4+-K+ co-loaded clinoptilolite as a binary fertilizer. Arch. Agron. Soil Sci., 66, 33-45,
Górska E.B., Maciejewska A., Jakubiak I., and Russel S., 2006. Effect of farm-yard manure, peat, brown coal and rekulter on occurrence of actinomycetes in soil (in Polish). Zeszyty Naukowe Uniwersytetu Przyrodniczego we Wrocławiu, 546, Rolnictwo, 89, 79-86.
Gul S., Whalen J.K., Thomas B.W., Sachdeva V., and Deng H.Y., 2015. Physico-chemical properties and microbial responses in biochar-amended soils: mechanisms and future directions. Agric. Ecosyst. Environ., 206, 46-59,
Houba V.J.G., Temminghoff E.J.M., Gaikhorst G.A., and van Vark W., 2000. Soil analysis procedures using 0.01 M calcium chloride as extraction reagent. Commun. Soil Sci. Plant Anal., 31 (9-10), 1299-1396,
Jarosz R., Szerement J., Gondek K., and Mierzwa-Hersztek M., 2022. The use of zeolites as an addition to fertilizers - a review. Catena, 213, 106125,
Khadem A. and Raiesi F., 2017. Responses of microbial performance and community to corn biochar in calcareous sandy and clayey soils. Appl. Soil Ecol., 114, 16-27,
Kizilkaya R., 2009. Nitrogen fixation capacity of Azotobacter spp. strains isolated from soils in different ecosystems and relationship between them and the microbiological properties of soils. J. Environ. Biol., 30, 73-82.
Kopeć M., Mierzwa-Hersztek M., Gondek K., Wolny-Koładka K., Zdaniewicz M., and Jarosz R., 2020. Biological activity of composts obtained from hop waste generated during the brewing. Biomass Conv. Bioref., 12, 1271-1279,
Kwiatkowska J., Provenzano M.R., and Senesi N., 2008. Long term effects of a brown coal-based amendment on the properties of soil humic acids. Geoderma, 148, 200-205,
Liu X., Guo K., Huang L., Ji Z., Jiang H., Li H., and Zhang J., 2017. Responses of absolute and specific enzyme activity to consecutive application of composted sewage sludge in a Fluventic Ustochrept. PLoS ONE, 12(5), e0177796,
Malinowski M., Wolny-Koładka K., and Vaverková M.D., 2019. Effect of biochar addition on the OFMSW composting process under real conditions. Waste Management, 84, 364-372,
Martinez-Toledo M.V., Moreno J., De la Rubia T., and Gonzalez-Lopez J., 1989. Root exudates of Zea mays and production of auxins, gibberellins and cytokinins by Azotobacter chroococcum. Plant Soil, 110, 149-152,
Martyniuk M. and Martyniuk S., 2003. Occurrence of Azotobacter spp. in some Polish soils. Pol. J. Environ. Stud., 12, 371-374.
Mikos-Szymańska M., Schab S., Rusek P., Borowik K., Bogusz P., and Wyzińska M., 2019. Preliminary study of a method for obtaining brown coal and biochar based granular compound fertilizer. Waste and Biomass Valorization, 10, 3673-3685,
Mierzwa-Hersztek M., Gondek K. Klimkowicz-Pawlas A., Chmiel M.J., Dziedzic K., and Taras H., 2019. Assessment of soil quality after biochar application based on enzymatic activity and microbial composition. Int. Agrophys., 33, 331-336,
Mierzwa-Hersztek M., Wolny-Koładka K., Gondek K., Gałązka A., and Gawryjołek K., 2020. Effect of coapplication of biochar and nutrients on microbiocenotic composition, dehydrogenase activity index and chemical properties of sandy soil. Waste and Biomass Valorization, 11, 3911-3923,
Mirzakhani M., Ardakani M.R., Aeene Band A., Rejali F., and Shirani Rad A.H., 2009. Response of spring safflower to co-inoculation with Azotobacter chroococum and Glomus intraradices under different levels of nitrogen and phosphorus. Am. J. Agricult. Biol. Sci., 4, 255-261,
Moreno J.L., Ondoño S., Torres I., and Bastida F., 2017. Compost, leonardite, and zeolite impacts on soil microbial community under barley crops. J. Soil Sci. Plant Nutr., 17(1), 214-230,
Natywa M., Sawicka A., and Wolna-Maruwka A., 2010. Microbial and enzymatic activity in the soil under maize crop in relation to differentiated nitrogen fertilisation. Water-Environment-Rural Areas, 10, 2(30), 111-120.
Neina Dora, 2019. The Role of soil pH in plant nutrition and soil remediation. App. Environ. Soil Sci., 1-9,
Oleszczuk N., Castro J.T., da Silva M.M., Korn M., Welz B., and Vale M.G., 2007. Method development for the determination of manganese, cobalt and copper in green coffee comparing direct solid sampling electrothermal atomic absorption spectrometry and inductively coupled plasma optical emission spectrometry. Talanta, 73(5), 862-869,
Oste L.A., Lexmond T.M., and Van Riemsdijk W.H., 2002. Metal immobilization in soils using synthetic zeolites. J. Environ. Qual., 31(3), 813-821,
Palleroni N.J., 1984. Gram negative aerobic rods and cocci. In: bergey’s manual of systematic bacteriology (Ed. N.R. Krieg), Williams &Wilkins, Baltimore, 140-199.
Ratanaprommanee C., Chinachanta K., Chaiwan F., and Shutsrirung A., 2016. Chemical characterization of leonardite and its potential use as soil conditioner and plant growth enhancement. Asia Pac. J. Sci. Technol., 22(4),1-10.
Rousk J., Brookes P.C., and Bååth E., 2009. Contrasting soil pH effects on fungal and bacterial growth suggest functional redundancy in carbon mineralization. Appl. Environ. Microbiol., 75(6), 1589-1596,
Rusyn I., Malovanyy M., Tymchuk I., and Synelnikov S., 2020. Effect of mineral fertilizer encapsulated with zeolite and polyethylene terephthalate on the soil microbiota, pH and plant germination. Ecol. Quest., 32, 1-12,
Sanli A., Karadogan T., and Tonguc M., 2013. Effects of leonardite applications on yield and some quality parameters of potatoes (Solanum tuberosum L.). Turkish J. Field Crops., 18(1), 20-26.
Santoso B., Cholid M., and Wijayanto R.A., 2022. Effect of zeolite and cow manure application on soil nitrogen content in ramie (Boehmeria nivea) plant growth. IOP Conf. Ser. Earth Environ. Sci., 974,
Shivakumara M.N., Krishna Murthy R., Subbarayappa C.T., Chamegowda T.C., Thimmegowda M.N., and Muthuraju R., 2019. Effect of zeolite and fertilizer application on soil microbial biomass and enzyme activity in finger millet. Int. J. Curr. Microbiol. App. Sci., 8, 1939-1957,
Szerement J., Szatanik-Kloc A., Jarosz R., Bajda T., Mierzwa-Hersztek M., 2021a. Contemporary applications of natural and synthetic zeolites from fly ash in agriculture and environmental protection. J. Clean. Prod., 311, 127461,
Szerement J., Szatanik-Kloc A., Mokrzycki J., and Mierzwa-Hersztek M., 2021b. The beneficial effects of agronomic biofortification with Se, Zn, and Fe on nutritional quality and stress defense of crops – a review. J. Soil Sci. Plant Nutr., 22, 1129-1159,
Thalmann A., 1968. Methods of dehydrogenase activity determination with triphenyl tetrazolium chloride (TTC) (in German). Landwirtsch. Forsch, 21, 249-258.
The Polish Committee for Standardization, 1998. Soils and mineral deposits – Sampling and determination of the grain size composition. PN-R-04032:1998 – (in Polish).
Tian J., Wang J., Dippold M., Gao Y., Blagodatskaya E., and Kuzyakov Y., 2016. Biochar affects soil organic matter cycling and microbial functions but does not alter microbial community structure in a paddy soil. Sci. Total Environ., 556, 89-97,
Tzanakakis V.A., Monokrousos N., and Chatzistathis T., 2021. Effects of clinoptilolite zeolite and vermiculite on nitrification and nitrogen and phosphorus acquiring enzymes in a nitrogen applied agricultural soil. J Soil Sci Plant Nutr., 21, 2791-2802,
Wolińska A., Zapasek M., and Stępniewska Z., 2016. The optimal TTC dose and its chemical reduction level. Acta Agroph., 23, 303-314.
Wolny-Koładka K. and Żukowski W., 2019. Mixed municipal solid waste hygienisation for refuse-derived fuel production by ozonation in the novel configuration using fluidized bed and horizontal reactor. Waste and Biomass Valorization, 10(3), 575-583,