RESEARCH PAPER
Influence of plant growth-promoting bacteria and green manures on the degree of weed infestation in Triticum spelta in organic farming
 
More details
Hide details
1
Institute of Agriculture and Horticulture, Faculty of Agricultural Sciences, University of Siedlce, Bolesława Prusa 14, 08-110 Siedlce, Poland
 
2
Faculty of Engineering and Economics, Ignacy Mościcki University of Applied Sciences in Ciechanów, Narutowicza 9, 06-400 Ciechanów, Poland
 
3
Department of Soil Science and Microbiology, Poznań University of Life Sciences, Szydłowska 50, 60-665 Poznań, Poland
 
These authors had equal contribution to this work
 
 
Final revision date: 2025-07-17
 
 
Acceptance date: 2025-09-04
 
 
Publication date: 2025-11-04
 
 
Corresponding author
Rafał Górski   

Faculty of Engineering and Economics, Ignacy Mościcki University of Applied Sciences in Ciechanów, Narutowicza 9, 06 - 400, Ciechanów, Poland
 
 
Int. Agrophys. 2026, 40(1): 1-11
 
HIGHLIGHTS
  • The use of bacterial consortia decreases the biomass of weeds
  • Ploughing of cover crops supports weed reduction
  • Bacterial consortia and green manures decrease dominant weeds
KEYWORDS
TOPICS
ABSTRACT
The research was conducted on an organic farm in Poland in 2019-2022. The purpose of the research was to determine the effect of plant growth-promoting rhizobacteria and cover crops plowed as green manure on the biomass, number, and species composition of weeds found in Triticum spelta grown in organic agriculture. Two factors were researched: I. Bacterial consortium (BC): control without the use of bacterial consortia; BC I: Bacillus subtilis + Bacillus amyloliquefaciens + Pseudomonas fluorescens; BC II: Bacillus subtilis + Bacillus amyloliquefaciens + Pseudomonas fluorescens + Azotobacter chroococum. II. Green manure: control without green manure; Trifolium pratense L.; Trifolium pratense L. + Lolium multiflorum L.; Lolium multiflorum L. The research was focused on the combined effect of plant growth-promoting bacteria with green manures on the degree of weed infestation in Triticum spelta grown in organic agriculture. The highest efficiency of weed control in Triticum spelta was demonstrated after application of BC II together with green manures from a mixture of Trifolium pratense with Lolium multiflorum and from Trifolium pratense.
FUNDING
This work was funded by the Ministry of Science and Higher Education Poland, grant number 29/20/B.
CONFLICT OF INTEREST
The Authors do not declare any conflict of interest.
REFERENCES (46)
1.
Abbas, T., Naveed, M., Siddique, S., Aziz, M.Z., Khan, K.S., Zhang, J.J., et al., 2020. Biological weeds control in rice (Oryza sativa) using beneficial plant growth promoting rhizobacteria. Int. J. Agric. Biol. 23, 552-558. https://doi.org/10.17957/IJAB/....
 
2.
Anderson, R.L., 2015. Suppressing weed growth after wheat harvest with underseeded red clover in organic farming. Renew. Agric. Food Syst. 31, 185-190. https://doi.org/10.1017/S17421....
 
3.
Artyszak, A., Gozdowski, D., 2021. Application of growth activators and plant growth-promoting rhizobacteria as a method of introducing a “farm to fork” strategy in crop management of winter oilseed. Sustainability 13, 3562. https://doi.org/10.3390/su1306....
 
4.
Baris, O., Sahin, F., Turan, M., Orhan, F., Gulluce, M., 2014. Use of plant-growth-promoting rhizobacteria (PGPR) seed inoculation as alternative fertilizer inputs in wheat and barley production. Commun. Soil Sci. Plant Anal. 45, 2457-2467. https://doi.org/10.1080/001036....
 
5.
Bhowmik, P.C., 2003. Challenges and opportunities in implementing allelopathy for natural weed management. Crop Prot. 22, 661-671. https://doi.org/10.1016/S0261-....
 
6.
Blaser, B.C., Singer, J.W., Gibson, L.R., 2011. Winter cereal canopy effect on cereal and interseeded legume productivity. Agron. J. 103, 1180-1185. https://doi.org/10.2134/agronj....
 
7.
Caamal-Maldonado, J.A., Jiménez-Osornio, J.J., Torres-Barragán, A., Anaya, A.L., 2001. The use of allelopathic legume cover and mulch species for weed control in cropping systems. Agron. J. 93, 27-36. https://doi.org/10.2134/agronj....
 
8.
Carlesi, S., Bigongiali, F., Antichi, D., Ciaccia, C., Tittarelli, F., Canali, S., et al., 2020. Green manure and phosphorus fertilization affect weed community composition and crop/weed competition in organic maize. Renew. Agric. Food Syst. 35, 493-502. https://doi.org/10.1017/S17421....
 
9.
Cinkocki, R., Lipková, N., Javoreková, S., Petrová, J., Maková, J., Medo, J., et al., 2021. The impact of growth-promoting streptomycetes isolated from rhizosphere and bulk soil on oilseed rape (Brassica napus L.) growth parameters. Sustainability 13, 5704. https://doi.org/10.3390/su1310....
 
10.
Dahiya, A., Sharma, R., Sindhu, S., Sindhu, S.S., 2019. Resource partitioning in the rhizosphere by inoculated Bacillus spp. towards growth stimulation of wheat and suppression of wild oat (Avena fatua L.) weed. Physiol. Mol. Biol. Plants 25, 1483-1495. https://doi.org/10.1007/s12298....
 
11.
Dar, A., Zahir, Z.A., Asghar, H.N., Ahmad, R., 2020. Preliminary screening of rhizobacteria for biocontrol of little seed canary grass (Phalaris minor Retz.) and wild oat (Avena fatua L.) in wheat. Can. J. Microbiol. 2020, 66, 368–376. https://doi.org/10.1139/cjm-20....
 
12.
Döring, T.F., Storkey, J., Baddeley, J.A., Collins, R.P., Crowley, O., Howlett, S.A., et al., 2017. Weeds in organic fertility-building leys: Aspects of species richness and weed management. Org. Farming 3, 51-65. https://doi.org/10.12924/of201....
 
13.
DuPre, M.E., Seipel, T., Bourgault, M., Boss, D.L., Menalled, F.D., 2022. Predicted climate conditions and cover crop composition modify weed communities in semiarid agroecosystems. Weed Res. 62, 38-48. https://doi.org/10.1111/wre.12....
 
14.
Frabboni, L., Tarantino, A., Petruzzi, F., Disciglio, G., 2019. Bio-herbicidal effects of oregano and rosemary essential oils on chamomile (Matricaria chamomilla L.) crop in organic farming system. Agronomy 9, 475. https://doi.org/10.3390/agrono....
 
15.
Glick, B.R., 2020. Beneficial Plant-Bacterial Interactions, 2nd ed. Springer: Berlin/Heidelberg, Germany, p. 383. https://doi.org/10.1007/978-3-....
 
16.
Górski, R., Rosa, R., Niewiadomska, A., Wolna-Maruwka, A., Głuchowska, K., Płaza, A., 2024. The use of a composition of bacterial consortia and living mulch to reduce weeds in organic spring barley cultivation as an element of sustainable plant production. Sustainability 16, 5268. https://doi.org/10.3390/su1612....
 
17.
Hashem, A., Tabassum, B., Fathi Abd Allah, E., 2019. Bacillus subtilis: A plant-growth promoting rhizobacterium that also impacts biotic stress. Saudi J. Biol. Sci. 26, 1291-1297. https://doi.org/10.1016/j.sjbs....
 
18.
Herrera, J.M., Rubio, G., Häner, L.L., Delgado, J.A., Lucho-Constantino, C.A., Islas-Valdez, S., et al., 2016. Emerging and established technologies to increase nitrogen use efficiency of cereals. Agronomy 6, 25. https://doi.org/10.3390/agrono....
 
19.
Kocira, A., Staniak, M., Tomaszewska, M., Kornas, R., Cymerman, J., Panasiewicz, K., et al., 2020. Legume cover crops as one of the elements of strategic weed management and soil quality improvement. Review. Agriculture 10, 394. https://doi.org/10.3390/agricu....
 
20.
Kosinski, M.S., King, J.R., Harker, K.N., Turkington, T.K., Spaner, D., 2011. Barley and triticale underseeded with a kura clover living mulch: Effects on weed pressure, disease incidence, silage yield, and forage quality. Can. J. Plant Sci. 91, 667-687. https://doi.org/10.4141/cjps10....
 
21.
Ladha, J.K., Peoples, M.B., Reddy, P.M., Biswas, J.C., Bennett, A., Jat, M.L., et al., 2022. Biological nitrogen fixation and prospects for ecological intensification in cereal-based cropping systems. Field Crops Res. 283, 108541. https://doi.org/10.1016/j.fcr.....
 
22.
Li, J., Kremer, R.J., 2006. Growth response of weed and crop seedlings to deleterious rhizobacteria. Biol. Control 39, 58-65. https://doi.org/10.1016/j.bioc....
 
23.
Liu, S., Ma, Z., Zhang, Y., Chen, Z., Du, X., Mu, Y., 2022. The impact of different winter cover crops on weed suppression and corn yield under different tillage systems. Agronomy 12, 999. https://doi.org/10.3390/agrono....
 
24.
Marcinkevičienė, A., Čmukas, A., Velička, R., Kosteckas, R., Skinulienė, L., 2023. Comparative analysis of undersown cover crops and bio-preparations on weed spread and organically grown spring oilseed rape yield. Sustainability 15, 13594. https://doi.org/10.3390/su1518....
 
25.
Minuț, M., Diaconu, M., Roșca, M., Cozma, P., Bulgariu, L., Gavrilescu, M., 2023. Screening of Azotobacter, Bacillus and Pseudomonas species as plant growth-promoting bacteria. Processes 11, 80. https://doi.org/10.3390/pr1101....
 
26.
Mishchenko, Y.G., Masik, I.M., 2017. Control of soil weediness and sugar beets by after crop green manure and different tillages. Ukr. J. Ecol. 7, 517-524. https://doi.org/10.15421/2017_....
 
27.
Mohler, C.L., Teasdale, J.R., 1993. Response of weed emergence to rate of Vicia villosa Roth and Secale cereale L. residue. Weed Res. 33, 487-499. https://doi.org/10.1111/j.1365....
 
28.
Moonen, A.C., Bàrberi, P., 2004. Size and composition of the weed seedbank after 7 years of different cover crop-maize management systems. Weed Res. 44, 163-177. https://doi.org/10.1111/j.1365....
 
29.
Mustafa, A., Naveed, M., Saeed, Q., Nadeem Ashraf, M., Hussain, A., Abbas, T., et al., 2019. Application potentials of plant growth promoting rhizobacteria and fungi as an alternative to conventional weed control methods. In Sustainable Crop Production; IntechOpen: London, UK. https://doi.org/10.5772/intech....
 
30.
Niewiadomska, A., Majchrzak, L., Borowiak, K., Wolna-Maruwka, A., Waraczewska, Z., Budka, A., et al., 2020. The influence of tillage and cover cropping on soil microbial parameters and spring wheat physiology. Agronomy 10, 200. https://doi.org/10.3390/agrono....
 
31.
Olanrewaju, O.S., Glick, B.R., Babalola, O.O., 2017. Mechanisms of action of plant growth promoting bacteria. World J. Microbiol. Biotechnol. 33, 197. https://doi.org/10.1007/s11274....
 
32.
Reed, L., Glick, B.R., 2023. The recent use of plant-growth-promoting bacteria to promote the growth of agricultural food crops. Agriculture 13, 1089. https://doi.org/10.3390/agricu....
 
33.
Restuccia, A., Scavo, A., Lombardo, S., Pandino, G., Fontanazza, S., Anastasi, U., et al., 2020. Long-term effect of cover crops on species abundance and diversity of weed flora. Plants 9, 1506. https://doi.org/10.3390/plants....
 
34.
Ranaldo, M., Carlesi, S., Costanzo, A., Barberi, P., 2019. Functional diversity of cover crop mixtures enhances biomass yield and weed suppression in a Mediterranean agroecosystem. Weed Res. 60, 96-108. https://doi.org/10.1111/wre.12....
 
35.
Ross, S.M., King, J.R., Izaurralde, R.C., O’Donovan, J.T., 2001. Weed suppression by seven clover species. Agron. J. 93, 820-827. https://doi.org/10.2134/agronj....
 
36.
Sati, D., Pande, V., Pandey, S.C., Samant, M., 2023. Recent advances in PGPR and molecular mechanisms involved in drought stress resistance. J. Soil Sci. Plant Nutr. 23, 106-124. https://doi.org/10.1007/s42729....
 
37.
Saucke, H., Ackermann, K., 2006. Weed suppression in mixed cropped grain peas and false flax (Camelina sativa). Weed Res. 46, 453-461. https://doi.org/10.1111/j.1365...
 
38.
Seidel, E.P., Caetano, J.H.S., Karpinski, A.S., Reis, W.D., 2019. Residual dry matter, weeds and soil aggregates after winter cover crop. J. Exp. Agric. Int. 32, 1-11. https://doi.org/10.9734/jeai/2....
 
39.
Seipel, T., Ishaq, S.L., Larson, C., Menalled, F.D., 2022. Weed communities in winter wheat: Responses to cropping systems under different climatic conditions. Sustainability 14, 6880. https://doi.org/10.3390/su1411....
 
40.
Sindhu, S.S., Khandelwal, A., Phour, M., Sehrawat, A., 2018. Bioherbicidal potential of rhizosphere microorganisms for ecofriendly weeds management. In Agriculturally Important Microbes for Sustainable Agriculture; Applications in Crop Production and Protection; Meena, V.S., Mishra, P.K., Bisht, J.K., Pattanayak, A., Eds, Springer: Singapore, 331-376. https://doi.org/10.1007/978-98....
 
41.
Sobczyk, A., Pycia, K., Jaworska, G., 2017. Comparative characteristic of technological value of grain of old varieties and new breeding lines of spelt (Triticum spelta L.) and grain of common wheat (Triticum vulgare). Zesz. Probl. Postępów Nauk Roln. 598, 81-91. https://doi.org/10.22630/ZPPNR....
 
42.
Teasdale, J.R., Mohler, C.L., 2000. The quantitative relationship between weed emergence and the physical properties of mulches. Weed Sci. 48, 385-392. https://doi.org/10.1614/0043-1....
 
43.
Weissmann, R., Uggla, C., Gerhardson, B., 2003. Field performance of a weed-suppressing Serratia plymuthica strain applied with conventional spraying equipment. BioControl 48, 725-742. https://doi.org/10.1023/A:1026....
 
44.
Wells, M.S., Reberg-Horton, S.C., Mirsky, S.B., 2016. Planting date impacts on soil water management, plant growth, and weeds in cover-crop-based no-till corn production. Agron. J. 108, 162-170. https://doi.org/10.2134/agronj....
 
45.
Xuan, T.D., Tawata, S., Khanh, T.D., Chung, I.M., 2005. Decomposition of allelopathic plants in soil. J. Agron. Crop Sci. 191, 162-171. http://dx.doi.org/10.1111/j.14....
 
46.
Yamane, K., Kono, M., Fukunaga, T., Iwai, K., Sekine, R., Watanabe, Y., et al., 2014. Field evaluation of coffee grounds application for crop growth enhancement, weed control, and soil improvement. Plant Prod. Sci. 17, 93-102. https://doi.org/10.1626/pps.17....
 
eISSN:2300-8725
ISSN:0236-8722
Journals System - logo
Scroll to top