Dynamic rheology and microstructure of starch gels affected by triticale genomic composition and developing stage
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Department of Food Research and Graduate Program (DIPA), University of Sonora, Hermosillo, Sonora 83000, Mexico
Laboratory of Biopolymers, CTAOA, Research Center for Food and Development, CIAD, A.C. Carretera a La Victoria Km. 0.6, Hermosillo, Sonora 83304, Mexico
Department of Polymers, University of Sonora, Col. Centro, Blvd. Luis Encinas y Rosales S/N, Hermosillo, Sonora, C.P. 83000, Mexico
Francisco J. Cinco-Moroyoqui   

Department of Food Research and Graduate Program (DIPA), University of Sonora, Hermosillo, Sonora 83000, Mexico
Publish date: 2019-02-06
Acceptance date: 2018-08-09
Int. Agrophys. 2019, 33(1): 21–30
Starches of developing triticale grains, differing in genome composition (complete AABBRR or substituted AABBDR), were evaluated in terms of starch granule distribution, dynamic rheological behaviour and microstructural characteristics on several days after anthesis. The starch granules were of an oblate spheroid shape for A-granules, and of a spherical shape for B-granules. However, those obtained from the complete triticale showed a larger diameter size. An X-ray diffraction analysis revealed the common A-type pattern of cereal starches from early development stages. A dynamic rheological analysis showed that the storage and loss moduli reached maximum levels in the temperature range of 71-86ºC and dropped at around 90ºC. Starches from the complete triticale showed lower phase transition temperatures, compared to those obtained from the substituted genotype (56.1±0.3 and 60.3±0.8°C, respectively). Scanning electron microscopy showed that the gels made with the starch of complete triticales were of a less dense sponge-like structure.
Alishahi A., Farahnaky A., Majzoobi M., and Blanchard C.L., 2015. Physicochemical and textural properties of corn starch gels: Effect of mixing speed and time. Food Hydrocoll., 45, 55-62.
Altenbach S.B., DuPont F.M., Kothari K.M., Chan R., Johnson E.L., and Lieu D., 2003. Temperature, water and fertilizer influence the timing of key events during grain development in a US spring wheat. J. Cereal Sci., 37, 9-20.
Ao Z. and Jane J., 2007. Characterization and modeling of the A- and B-granule starches of wheat, triticale, and barley. Carbohyd. Polym., 67, 46-55.
Bechtel D.B., Zayas I., Kaleikau L., and Pomeranz Y., 1990. Size distribution of wheat starch granules during endosperm development. Cereal Chem., 67, 59-63.
Bettge A.D., Morris C.F., and Greenblatt G.A., 1995. Assessing genotypic softness in single wheat kernels using starch granule-associated friabilin as a biochemical marker. Euphytica, 86, 65-72.
Cao Y., Hu W., and Wang C., 2012. Relationship among the key enzymatic activities involved in starch synthesis and amylopectin chain distributions in developing wheat grain. Afr. J. Biotechnol, 11(4), 805-814.
Cheetham N.W.H. and Tao L., 1998. Variation in crystalline type with amylose content in maize starch granules: an X-ray powder diffraction study. Carbohyd. Polym., 36(4), 277-284.
Chung H.J. and Liu Q., 2009. Impact of molecular structure of amylopectin and amylose on amylose chain association during cooling. Carbohyd. Polym., 77(4), 807-815.
Copeland L., Blazek J., Salman H., and Tang M.C., 2009. Form and functionality of starch. Food Hydrocoll., 23(6), 1527-1534.
Cornejo-Ramírez Y.I., Cinco-Moroyoqui F.J., Ramírez-Reyes F., Rosas-Burgos E.C., Osuna-Amarillas P.S., Wong-Corral F.J., Borboa-Flores J., and Cota-Gastélum A.G., 2015. Physicochemical characterization of starch from hexaploid triticale (X Triticosecale Wittmack) genotypes. CyTA J. Food, 13(3), 420-426.
Cornejo-Ramírez Y.I., Ramírez-Reyes F., Cinco-Moroyoqui F.J., Rosas-Burgos E.C., Martínez-Cruz O., Carvajal-Millán E., Cárdenas-López J.L., and Wong-Corral F., 2016. Starch debranching enzyme activity and its effects on some starch physicochemical characteristics in developing substituted and complete triticales (X Triticosecale Wittmack). Cereal Chem., 93(1), 64-70.
Finnie S.M., Jeannotte R., and Faubion J.M., 2009. Quantitative characterization of polar lipids from wheat whole meal, flour, and starch. Cereal Chem., 86(6), 637-645.
Hayakawa K., Tanaka K., Nakamura T., Endo S., and Hoshino T., 1997. Quality characteristics of waxy hexaploid wheat (Triticum aestivum L.): properties of starch gelatinization and retrogradation. Cereal Chem., 74, 576-580.
Hizukuri S., Takeda Y., Yasuda M., and Suzuki A., 1981. Multi-branched nature of amylose and the action of debranching enzymes. Carbohyd. Res., 94(2), 205-213.
Inoue M. and Hirasawa I., 2013. The relationship between crystal morphology and XRD peak intensity on CaSO4 × 2H2O. J. Cryst. Growth, 380, 169-175.
Jane J., Chen Y.Y., Lee L.F., McPherson A.E., Wong K.S., Radosavljevic M., and Kasemsuwan T., 1999. Effects of amylopectin branch chain length and amylose content on the gelatinization and pasting properties of starch 1. Cereal Chem., 76(5), 629-637.
Jane J., Kasemsuwan T., Leas S., Zobel H. and Robyt J.F., 1994. Anthology of starch granule morphology by scanning electron microscopy. Starch-Stärke, 46, 121-129.
Jeon J.S., Ryoo N., Hahn T.R., Walia H., and Nakamura Y., 2010. Starch biosynthesis in cereal endosperm. Plant Physiol. Bioch., 48(6), 383-392.
Jiamjariyatam R., Kongpensook V., and Pradipasena P., 2015. Effects of amylose content, cooling rate and aging time on properties and characteristics of rice starch gels and puffed products. J. Cereal Sci., 61, 16-25.
Kaur L., Singh J., Singh H., and McCarthy O.J., 2008. Starch-cassia gum interactions: A microstructure-rheology study. Food Chem, 111(1), 1-10.
Kim J., Zhang C., and Shin M., 2015. Forming rice starch gels by adding retrograded and cross-linked resistant starch prepared from rice starch. Food Sci. Biotechnol., 24(3), 835-841.
Kim W., Johnson J.W., Graybosch R.A., and Gaines C.S., 2003. Physicochemical properties and end-use quality of wheat starch as a function of waxy protein alleles. J. Cereal Sci., 37(2), 195-204.
Mellado M.Z., Matus I.T., and Madariaga R.B., 2008. Background on triticale in Chile and other countries. Bulletin INIA, 183, 1-75.
Sang Y., Bean S., Seib P.A., Pedersen J., and Shi Y.C., 2008. Structure and functional properties of sorghum starches differing in amylose content. J. Agric. Food Chem., 56, 6680-6685.
SAS Institute, 2005. PROC user’s manual, 8th version. Cary, NC: SAS Institute. Sasaki T., Yasui T., and Matsuki J., 2000. Effect of amylose content on gelatinization, retrogradation, and pasting properties of starches from waxy and nonwaxy wheat and their F1 seeds. Cereal Chem., 77(1), 58-63.
Singh N., Singh J., Kaur L., Sodhi N.S., and Gill B.S., 2003. Morphological, thermal and rheological properties of starches from different botanical sources. Food Chem., 81(2), 219-231.
Song Y. and Jane J., 2000. Characterization of barley starches of waxy, normal, and high amylose varieties. Carbohyd.Polym., 41, 365-377.
Sun Q., Wu M., Bu X., and Xiong L., 2015. Effect of the amount and particle size of wheat fiber on the physicochemical properties and gel morphology of starches. PLoS ONE 10(6): e0128665.
Ulbrich M., Wiesner I., and Flöter E., 2015. Molecular characterization of acid-thinned wheat, potato and pea starches and correlation to gel properties. Starch-Stärke, 67(5-6), 424-437.
Varughese G., Barker T., and Saari E., 1987. Triticale. CIMMYT, México, DF, pp. 32.
Yamin F.F., Lee M., Pollak L.M., and White P.J., 1999. Thermal properties of starch in corn variants isolated after chemical mutagenesis of inbred line B73. Cereal Chem., 76(2), 175-181.
Yoo S. and Jane J., 2002. Structural and physical characteristics of waxy and other wheat starches. Carbohyd. Polym., 49, 297-305.
Zhang H., Zhang W., Xu C., and Zhou X., 2013. Morphological features and physicochemical properties of waxy wheat starch. Int. J. Biol. Macromol., 62, 304-309.
Zhao H.J., Zou Q., and Zhang X.Y., 2003. Comparison between two wheat varieties with different spike type in carbohydrate metabolism during late growth period. Acta Agron. Sinica, 29(5), 676-681.