Optimization of acid assisted extraction process of foxtail millet polysaccharides and its antioxidant activity
More details
Hide details
Zhejiang University of Science and Technology, China
Final revision date: 2019-12-20
Acceptance date: 2020-01-26
Publication date: 2020-02-25
Corresponding author
Aishi Zhu   

Zhejiang University of Science and Technology, Zhejiang University of Science and Technology, 318 Liuhe Road, 310023, Hangzhou, China
Int. Agrophys. 2020, 34(2): 141-149
The hydrochloric acid assisted extraction experiment of polysaccharides from foxtail millet was carried out. The extraction condition combination of hydrochloric acid concentration, liquid-solid ratio, extraction time and extraction temperature was investigated by Response Surface Methodology of three levels and four variable Box–Behnken designs to obtain the maximum yield of polysaccharides. The experimental data were fitted to a quadratic equation using multiple regression analysis and analyzed. The antioxidant activity of obtained polysaccharides was meanwhile investigated in vitro. The optimum conditions were acid concentration 2.4 mol L-1, liquid-solid ratio 19.3:1 mL g-1, extraction time 1.2 h, extraction temperature 81.0 °C. Under these conditions, the experimental yield was 52.17 mg g-1, which was well in close agreement with 52.39 mg g-1 predicted value by the model. The mathematical model had high correlation (p<0.05). The polysaccharides of foxtail millet have better capacity of radical scavenging activities on superoxide and hydroxyl radical-scavenging activity.
Bendahou A., Dufresne A., Kaddami H., and Habibi Y., 2007. Isolation and structural characterization of hemicelluloses from palm of phoenix Dactylifera L. Carbohydrate Polymers, 68(3): 601-608.
Chen Y., Xie M.Y., and Gong X.F., 2007. Microwave-assisted extraction used for the isolation of total triterpenoid saponins from Ganoderma atrum. J. Food Eng., 81(1), 162-170.
Gan C.Y., Abdul Manaf N., and Latiff A.A., 2010. Optimization of alcohol insoluble polysaccharides (AIPS) Extraction from the parkia speciosa pod using response surface methodology (RSM). Carbohydrate Polymers, 79(4): 825-831.
Guo L., Guo J.C., Zhu W.C., and Jiang X.R., 2016. Optimized synchronous extraction process of tea polyphenols and polysaccharides from huaguoshan yunwu tea and their antioxidant activities. Food Bioproducts Processing, 100: 303-310.
Lei Z.T., Chen H., Huang D., Zhai Y.L., and Shu G.W., 2016. Optimization of medium compositions for saccharomyces boulardii by box-behnken design. Scientific Study Res.Chemistry Chemical Eng., Biotechnol., Food Industry, 17(4): 405-416.
Li S.H., Wang L.B., Song C.F., Hu X.S., Sun H.Y., Yang Y.N., Lei Z.F., and Zhang Z.Y., 2014. Utilization of soybean curd residue for polysaccharides by Wolfiporia extensa (Peck) Ginns and the antioxidant activities in vitro. J.Taiwan Institute of Chemical Engineers, 45: 6-11.
Maran J.P., Manikandan S., Thirugnanasambandham K., Nivetha C.V., and Dinesh R., 2013. Box-behnken design based statistical modeling for ultrasound-assisted extraction of corn silk polysaccharide. Carbohydrate Polymers, 92(1): 604-611.
Osman H., Shigidi I., and Elkhaleefa A., 2016. Optimization of sesame seeds oil extraction operating conditions using the response surface design methodology. Scientific Study Research Chemistry Chemical Eng., Biotechnol., Food Industry, 17(4): 335-347.
Quan Y., Yang S., Wan J., Su T.T., Zhang J., and Wang Z.Y., 2015. Optimization for the extraction of polysaccharides from nostoc commune and its antioxidant and antibacterial activities. J. Taiwan Institute of Chemical Engineers, 52: 14-21.
Radhika G.B., Satyanarayana S.V., and Rao D.G., 2011. Mathematical model on thin layer drying of finger millet (Eluesine coracana). Advance J. Food Sci. Technol., 3(2): 127-131.
Ren C.J., Gao L., Wang C.M., Wu M.C., and Chen J.F., 2008. Optimization of extracting technology of hydrosoluble polysaccharides from peanut meal by alkali. Trans. CSAE, 24(7): 251-254.
Tian S.Y., Hao C.C., Xu G.K., Yang J.J., and Sun R.G., 2017. Optimization conditions for extracting polysaccharide from angelica sinensis and its antioxidant activities. J. Food Drug Analysis, 25(4): 766-775.
Wang C.Y., Wang Y., Zhang J., and Wang Z.Y., 2014a. Optimization for the extraction of polysaccharides from Gentiana scabra Bunge and their antioxidant in vitro and anti-tumor activity in vivo. J. Taiwan Institute of Chemical Engineers, 45(4): 1126-1132.
Wang P.R., Chen D.F., Jiang D.H., Dong X.M., Chen P.P., and Lin Y.X., 2014b. Alkali extraction and in vitro antioxidant activity of monascus mycelium polysaccharides. J. Food Sci. Technol., 51(7): 1251-1259.
Yaich H., Garna H., Besbes S., Paquot M., Blecker C., and Attia H., 2013. Effect of extraction conditions on the yield and purity of ulvan extracted from Ulva lactuca. Food Hydrocolloids, 31(2): 375-382.
Yuan Y., Liu Y., Liu M.D., Chen Q., Jiao Y.Y., Liu Y., and Meng Z.L., 2017. Optimization extraction and bioactivities of polysaccharide from wild Russula griseocarnosa. Saudi Pharmaceutical J., 25(4): 523-530.
Zeng H.L., Zhang Y., Lin S., Jian Y.Y., Miao S., and Zheng B.D., 2015. Ultrasonic-microwave synergistic extraction (UMSE) and molecular weight distribution of polysaccharides from Fortunella margarita (Lour.) swingle. Separation and Purification Technol., 144: 97-106.
Zhang X.H., Liu L.N., and Lin C.W., 2014. Isolation, structural characterization and antioxidant activity of a neutral polysaccharide from Sisal waste. Food Hydrocolloids, 39: 10-18.
Zhao B.T., Zhang J., Guo X., and Wang J.L., 2013. Microwave-assisted extraction, chemical characterization of polysaccharides from Lilium davidii var. unicolor salisb and its antioxidant activities evaluation. Food Hydrocolloids, 31(2): 346-356.
Zhu A.S., Xu M.Q., and Zhao J.Z., 2017. Alkali extraction of polysaccharides from proso millet and antioxidant activity. Scientific Study Res. Chemistry Chemical Eng., Biotechnol., Food Industry, 18(4): 353-365.
Zhu A.S., Ye J.N., and Yan F.N., 2014. Optimization of extraction technology of polysaccharide from foxtail millet using response surface methodology. Chemical Industry Chemical Eng. Quarterly, 20(4): 579-585.
Journals System - logo
Scroll to top