Use of saccharose and structural polysaccharides from sugar beet biomass for bioethanol production
More details
Hide details
Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Poland
Final revision date: 2019-12-03
Acceptance date: 2020-01-13
Publication date: 2020-02-28
Corresponding author
Maria Balcerek   

Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Poland
Int. Agrophys. 2020, 34(2): 151-159
In addition to saccharose, sugar beet root contains a lignocellulosic fraction, which is not used in the process of sugar production and remains in sugar beet pulp. There is a great interest in using the polysaccharides (cellulose, hemicellulose) present in this raw material for the production of bioethanol. The objective of this study was to assess the effect of the enzymatic treatment of sugar beet biomass on the hydrolysis of the cellulose and hemicellulose present in its cell walls, as well as its effect on the efficiency of alcoholic fermentation of saccharose and sugars liberated from structural polysaccharides. Its effect on the efficiency of the process of inoculating the fermentation medium with a monoculture or a co-culture of yeast strains fermenting hexose and pentose sugars was also investigated. Our results reveal that in order to enable the utilization of all fermentable sugars in the sugar beet root biomass (saccharose as well as monosaccharides bound in structural polysaccharides), initial enzymatic treatment should be applied, followed by alcoholic fermentation using sequential inoculation with a co-culture of Saccharomyces cerevisiae and Pichia stipitis. These conditions ensure the utilization of hexoses and pentoses (xylose) in alcoholic fermentation, thus enabling the production of 9.9±0.4 kg of ethanol from 100 kg of sugar beet biomass.
Alvira P., Tomás-Pejó E., Ballesteros M., and Negro M.J., 2010. Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: a review. Biores. Technol., 101, 4851-4861.
AOAC Official Methods of Analysis of AOAC International, 1995. Vol. 2, AOAC Int., MD, USA,.
Arasimovich V.V. and Ermakov A.I., 1987. Measurement of the total content of hemicelluloses. In: Methods for Biochemical Studies of Plants (Ed. A.I. Ermakov). Agropromizdat, Saint Petersburg, Russia.
Bai F.W., Anderson W.A., and Moo-Young M., 2008. Ethanol fermentation technologies from sugar and starch feedstocks. Biotechnol. Adv., 26, 89-105.
Batt C.A., Carvallo S., Easson D.D., Akado M., and Sinskey A.J., 1986. Direct evidence for a xylose metabolic pathway in Saccharomyces cerevisiae. Biotechnol. Bioeng., 28, 549-553.
Berłowska J., Pielech-Przybylska K., Balcerek M., Dziekońska-Kubczak U., Patelski P., Dziugan P., and Kręgiel D., 2016. Simultaneous saccharification and fermentation of sugar beet pulp for efficient bioethanol production. BioMed Res., Article ID 3154929.
du Preez J.C., Bosch M., and Prior B.A., 1986. Xylose fermentation by Candida shehatae and Pichia stipitis: effects of pH, temperature and substrate concentration. Enzyme Microb. Technol., 8(6), 360-364.
Dziugan P., Balcerek M., Pielech-Przybylska K., and Patelski P., 2013. Evaluation of the fermentation of high gravity thick sugar beet juice worts for efficient bioethanol production. Biotechnol. Biofuels, 6, 158.
European Seed Association (ESA), 2019. Available from:
García-Cubero M.T., González-Benito G., Indacoechea I., Coca M., and Bolado S., 2009. Effect of ozonolysis pretreatment on enzymatic digestibility of wheat and rye straw. Biores. Technol., 100(4), 1608-1613.
Gong C.S., 1983. Recent advances in D-xylose conversion by yeasts. Annual Reports of Fermentation Processes, 6, 253-297.
Gumienna M., Szambelan K., Jeleń H., and Czarnecki Z., 2014. Evaluation of ethanol fermentation parameters for bioethanol production from sugar beet pulp and juice. J. Inst. Brew., 120, 543-549.
Kürschner K. and Hoffer A., 1933. Cellulose and cellulose derivatives. Fresenius’ J. Anal. Chem., 92(3), 145-154.
Kühnel S., Schols H.A., Gruppen H., 2011. Aiming for the complete utilization of sugar-beet pulp: Examination of the effects of mild acid and hydrothermal pretreatment followed by enzymatic digestion. Biotechnol. Biofuels, 4, 14.
Lovegrove A., Edwards C.H., De Noni I., Patel H., El S.N., Grassby T., Zielke C., Ulmius M., Nilsson L., Butterworth P.J., Ellis P.R., and Shewry P.R., 2015. Role of polysaccharides in food, digestion, and health. Crit. Rev. Food Sci. Nutr., 57(2), 237-253.
Micard V., Renard C.M., and Thibault J.F., 1996. Enzymatic saccharification of sugar-beet pulp. Enzyme Microb. Technol., 19, 162-170.
Miller G.L., 1959. Use of dinitrosalicylic acid reagent for determining reducing sugars. Anal. Chem., 31, 426-428.
Pessoa A., de Mancilha I.M., and Sato S., 1996. Cultivation of Candida tropicalis in sugar cane hemicellulosic hydrolyzate for microbial protein production. J. Biotechnol., 5, 83-88.
Pessoa A., de Mancilha I.M., and Sato S., 1997. Evaluation of sugar cane hemicellulose hydrolyzate for cultivation of yeasts and filamentous fungi. J. Ind. Microbiol. Biotechnol., 18, 360-363.
Rouhollah H., Iraj N., Giti E., and Sora A., 2007. Mixed sugar fermentation by Pichia stipitis, Sacharomyces cerevisiae, and an isolated xylose fermenting Kluyveromyces marxianus and their co-cultures. Afr. J. Biotechnol., 6, 1110-1114.
SUGAR NEWS & REPORTS, 2019. Available at:
Templeton D. and Ehrman T., 1995. Determination of acid-insoluble lignin in biomass. Chemical Analysis and Testing Task, Laboratory Analytical Procedure LAP-003. National Renewable Energy Laboratory (NREL), Golden, CO.
Wolak P. and Złocińska A., 2012. Examination of the chemical composition of sugar beet pulp – a by-product of sugar industry (in Polish). Eng. Sci. Technol., 2, 109-119.
Journals System - logo
Scroll to top