Optimization of acid assisted extraction process of foxtail millet polysaccharides and its antioxidant activity
Yanwen Shi 1
Aishi Zhu 1  
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Zhejiang University of Science and Technology, China
Aishi Zhu   

Zhejiang University of Science and Technology, Zhejiang University of Science and Technology, 318 Liuhe Road, 310023, Hangzhou, China
Publication date: 2020-02-25
Final revision date: 2019-12-20
Acceptance date: 2020-01-26
Int. Agrophys. 2020, 34(2): 141–149
The hydrochloric acid assisted extraction experiment of polysaccharides from foxtail millet was carried out. The extraction condition combination of hydrochloric acid concentration, liquid-solid ratio, extraction time and extraction temperature was investigated by Response Surface Methodology of three levels and four variable Box–Behnken designs to obtain the maximum yield of polysaccharides. The experimental data were fitted to a quadratic equation using multiple regression analysis and analyzed. The antioxidant activity of obtained polysaccharides was meanwhile investigated in vitro. The optimum conditions were acid concentration 2.4 mol L-1, liquid-solid ratio 19.3:1 mL g-1, extraction time 1.2 h, extraction temperature 81.0 °C. Under these conditions, the experimental yield was 52.17 mg g-1, which was well in close agreement with 52.39 mg g-1 predicted value by the model. The mathematical model had high correlation (p<0.05). The polysaccharides of foxtail millet have better capacity of radical scavenging activities on superoxide and hydroxyl radical-scavenging activity.
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