SHORT NOTE
Comparison of commonly used extraction methods for ergosterol in soil samples
| 1 | Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences, Institute of Soil Research, Vienna, Austria |
| 2 | Faculty of Agricultural Technology, Universitas Gadjah Mada, Departement of Agricultural and Biosystem Engineering, Yogyakarta, Indonesia |
| 3 | Department of Crop Sciences, University of Natural Resources and Life Sciences, Institute of Agronomy, Tulln an der Donau, Austria |
CORRESPONDING AUTHOR
Orracha Sae-Tun
Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences, Institute of Soil Research, 1190, Vienna, Austria
Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences, Institute of Soil Research, 1190, Vienna, Austria
Final revision date: 2020-09-08
Acceptance date: 2020-09-21
Publication date: 2020-10-19
Int. Agrophys. 2020, 34(4): 425–432
KEYWORDS
TOPICS
ABSTRACT
Concerning the contribution of fungi to soil carbon sequestration, various methods have been used to extract ergosterol from soil samples. This study aims to explore the extraction ability and applicability of commonly used methods to extract ergosterol from two contrasting soils. An agricultural soil (chernozem) and a forest soil (podzol) were extracted with different types of cell lysis such as alkaline, glass bead, and ultrasonication methods in association with simple shaking. The ergosterol concentration was measured by high pressure liquid chromatography. Regardless of the method applied, ergosterol yield was higher in podzol than in chernozem. Alkaline extraction resulted in the highest ergosterol concentrations for both soils and miniaturized glass bead extraction produced comparable results in the case of chernozem. In terms of applicability, the non-alkaline methods were simpler to conduct and less demanding of labour, chemical use and glassware and more flexible in terms of the equipment used for mechanical disruption. Despite the limit of the two soil types in the present study, only the simple shaking method was revealed to be dependent on soil type. Based on our results, we recommend the miniaturized glass bead method for agricultural soils, low in organic matter for high throughput. However, not all of the methods described allow for the proper separation of co-extracted organic substances from organic-rich soil.
REFERENCES (30)
1.
Ananyeva N.D., Susyan E. A., Chernova O.V., Chernov I.Yu., and Makarova O.L., 2006. The ratio of fungi and bacteria in the biomass of different types of soil determined by selective inhibition. Microbiology, 75, 702-107. https://doi.org/10.1134/s00262....
2.
Bååth E. and Anderson T.H., 2003. Comparison of soil fungal/bacterial ratios in a ph gradient using physiological and PLFA-Based Techniques. Soil Biol. Biochem., 35, 955-963.https://doi.org/10.1016/s0038-....
3.
Bååth E., 2001. Estimation of fungal growth rates in soil using 14C-Acetate Incorporation into ergosterol. Soil Biol. Biochem., 33, 2011-2018. https://doi.org/10.1016/s0038-....
4.
Beni A., Soki E., Lajtha K., and Fekete I., 2014. An Optimized HPLC method for soil fungal biomass determination and its application to a detritus manipulation study. J. Microbiol. Methods, 103, 124-130. https://doi.org/10.1016/j.mime....
5.
Bhople P., Djukic I., Keiblinger K., Zehetner F., Liu D., Bierbaumer M., Zechmeister-Boltenstern S., Joergensen R.G., and Murugan R., 2019. Variations in soil and microbial biomass C, N and fungal biomass ergosterol along elevation and depth gradients in alpine ecosystems. Geoderma, 345, 93-103. https://doi.org/10.1016/j.geod....
6.
Chiocchio V.M. and Matković L., 2011. Determination of ergosterol in cellular fungi by HPLC. A modified technique. J. Argentine Chemical Society, 98, 10-15.
7.
Corá J.E., Fernandes C., Beraldo J.M.G., and Marcelo A.V.M., 2009. Use of sand for soil dispersion in granulometric analysis. Revista Brasileira de Ciência do Solo, 33, 255-262. https://doi.org/10.1590/s0100-....
8.
Djajakirana G., Joergensen R.G., and Meyer B., 1996. Ergosterol and microbial biomass relationship in soil. Biology Fertility of Soils, 22, 299-304. https://doi.org/10.1007/s00374....
9.
Eash N.S., Stahl P.D., Parkin T.B., and Karlen D.L., 1996. A simplified method for extraction of ergosterol from Soil. Soil Science Society of America J., 60, 468-471. https://doi.org/10.2136/sssaj1....
10.
Feeney D.S., Crawford J.W., Daniell T., Hallett P.D., Nunan N., Ritz K., Rivers M., and Young I.M., 2006. Three-dimensional microorganization of the soil-root-microbe system. Microbial Ecology, 52, 151-158. https://doi.org/10.1007/s00248....
11.
Gong P., Guan X., and Witter E., 2001. A rapid method to extract ergosterol from soil by physical disruption. Applied Soil Ecology, 17, 285-89. https://doi.org/10.1016/s0929-....
12.
van Groenigen K.J., Bloem J., Bååth E., Boeckx P., Rousk J., Bodé S., Forristal D., and Jones M.B., 2010. Abundance, production and stabilization of microbial biomass under conventional and reduced tillage. Soil Biol. Biochem., 42, 48-55. https://doi.org/10.1016/j.soil....
13.
Joergensen R.G. and Wichern F., 2008. Quantitative assessment of the fungal contribution to microbial tissue in soil. Soil Biol. Biochem., 40, 2977-2991. https://doi.org/10.1016/j.soil....
14.
Mayer H., Mentler A., Papakyriacou M., Rampazzo N., Marxer Y., and Blum W.E.H., 2002. Influence of vibration amplitude on the ultrasonic dispersion of soils. Int. Agrophysics, 16, 53-60.
15.
Mentler A., Mayer H., Strauß P., and Blum W.E.H., 2004. Characterisation of soil aggregate stability by ultrasonic dispersion. Int. Agrophysics, 18, 39-45.
16.
Newell S.Y., Arsuffi T.L., and Fallon R.D., 1988. Fundamental procedures for determining ergosterol content of decaying plant material by liquid chromatography. Applied Environ. Microbiol., 54, 1876-1879. https://doi.org/10.1128/aem.54....
17.
Nikodem A., Pavlů L., Kodešová R., Borůvka L., and Drábek O., 2013. Study of podzolization process under different vegetation cover in the Jizerské hory Mts. Region. Soil Water Res., 8, 1-12. https://doi.org/10.17221/56/20....
18.
de Ridder-Duine A.S., Smant W., van der Wal A., van Veen J.A., and de Boer W., 2006. Evaluation of a simple, non-alkaline extraction protocol to quantify soil ergosterol. Pedobiologia, 50, 293-300. https://doi.org/10.1016/j.pedo....
19.
R Core Team, 2020. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. URL https://www.R-project.org/.
20.
Rousk J. and Bååth E., 2011. Growth of saprotrophic fungi and bacteria in soil. FEMS Microbiol. Ecology, 78, 17-30. https://doi.org/10.1111/j.1574....
21.
Ruzicka S., Norman M.D.P., and Harris J.A., 1995. Rapid ultrasonication method to determine ergosterol concentration in soil. Soil Biol. Biochem., 27, 1215-1217. https://doi.org/10.1016/0038-0....
22.
Ruzicka S., Edgerton D., Norman M., and Hill T., 2000. The utility of ergosterol as a bioindicator of fungi in temperate soils. Soil Biol. Biochem., 32, 989-1005. https://doi.org/10.1016/s0038-....
23.
Schomakers J., Zehetner F., Mentler A., Ottner F., and Mayer H., 2015. Study of soil aggregate breakdown dynamics under low dispersive ultrasonic energies with sedimentation and X-ray attenuation. Int. Agrophys., 29, 501-508. https://doi.org/10.1515/intag-....
24.
Seitz L.M., Mohr H.E., Burroughs R., and Sauer D.B., 1977. Ergosterol as an indicator of fungal invasion in grains. Cereal Chemistry, 54, 1207-1217.
25.
Strickland M.S. and Rousk J., 2010. Considering fungal: bacterial dominance in soils - methods, controls, and ecosystem implications. Soil Biol. Biochem., 42, 1385-1395. https://doi.org/10.1016/j.soil....
26.
Turgay O.C. and Nonaka M., 2002. Effects of land-use and management practices on soil ergosterol content in andosols. Soil Sci. Plant Nutrition, 48, 693-99. https://doi.org/10.1080/003807....
27.
Verma B., Robarts R.D., Headley J.V., Peru K.M., and Christofi N., 2002. Extraction efficiencies and determination of ergosterol in a variety of environmental matrices. Communications in Soil Science and Plant Analysis, 33, 3261-3275. https://doi.org/10.1081/css-12....
28.
Wallander H., Ekblad A., Godbold D.L., Johnson D., Bahr A., Baldrian P., Björk R.G., Kieliszewska-Rokicka B., Kjøller R., Kraigher H., Plassard C., and Rudawska M., 2013. Evaluation of methods to estimate production, biomass and turnover of ectomycorrhizal mycelium in forests soils - A Review. Soil Biol. Biochem., 57, 1034-1047. https://doi.org/10.1016/j.soil....
29.
Young I.M., Illian J., Harris J.A., and Ritz K., 2006. Comment on Zhao et al. (2005) Does ergosterol concentration provide a reliable estimate of soil fungal biomass? Soil Biol. Biochem., 38, 1500-1501. https://doi.org/10.1016/j.soil....
30.
Zhao X.R., Lin Q., and Brookes P.C., 2005. Does soil ergosterol concentration provide a reliable estimate of soil fungal biomass? Soil Biol. Biochem., 37, 311-317. https://doi.org/10.1016/j.soil....
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