Field effectiveness of gaseous ozone storage in alleviating damage to aged soybean seeds
More details
Hide details
Research Center for Food Technology and Processing, National Research and Innovation Agency, Jl. Yogya-Wonosari KM 31.5, Gading, Playen Gunung Kidul, Yogyakarta 55861, Indonesia
Research Center for Horticultural and Estate Crops, National Research and Innovation Agency, Jl. Raya Bogor-Jakarta, Cibinong, Bogor 16911, Indonesia
Research Center for Food Crops, National Research and Innovation Agency, Jl. Raya Bogor-Jakarta, Cibinong, Bogor 16911, Indonesia
Research Center for Behavioral and Circular Economics, National Research and Innovation Agency, Jl. Jend. Gatot Subroto No.10, Jakarta 12710, Indonesia
Research Center for Sustainable Production System and Life Cycle Assessment, National Research and Innovation Agency, Gedung Pusat Inovasi dan Bisnis Teknologi No.720, Kawasan Puspitek Serpong, Tangerang Selatan 15314, Indonesia
Physics Department, Faculty of Science and Mathematics, Diponegoro University, Jl. Prof. Soedarto, Tembalang, Semarang 50275, Indonesia
Final revision date: 2023-09-21
Acceptance date: 2023-10-03
Publication date: 2023-10-31
Corresponding author
Indrie Ambarsari   

Research Center for Food Technology and Processing, National Research and Innovation Agency, Indonesia
Int. Agrophys. 2023, 37(4): 435-441
  • Ozone effectively extends the longevity of aged soybean seeds up to five months by increasing their viability;
  • Ozone triggers the antioxidant defence system and healing mechanism in aged soybean seeds;
  • The aged seed viability increased in ozone storage contributed to reducing seed losses and financial losses.
Ozonation is a promising method for maintaining seed quality. However, the ability of ozone to preserve the quality of aged seeds, which have already deteriorated, is still open to question. This study evaluated the effectiveness of ozone in alleviating ageing soybean seed damage and also the economic feasibility of ozone technology under field conditions. Samples under ozonation storage (they were stored in open containers and woven sacks) were compared with those stored under conventional storage conditions (they were stored in woven bags without ozonation). Ozonated samples were exposed to 150 g h–1 of ozone gas for three hours a day for a six month storage period. All samples (with and without ozonation) were stored at 18±5°C and 50±5% relative humidity. Observations were conducted every month with regard to the seeds germination percentage, vigour index, moisture content, protein and free fatty acids. The results showed that ozonation has the potential to increase the viability of aged soybean seeds for up to 5 months. However, the decrease in the protein and free fatty acids levels in the ozonated seeds occurred more rapidly than in the control. Moreover, no significant difference was found between the ozonated seeds in open containers and sacks during storage, except for their moisture content. Ozone application on aged soybean seeds was found to be financially feasible with an ratio of revenue to cost value of 1.73, an return on investment of 74.63% and a payback period of 1.34 years.
The authors declare that there is no conflict of interest regarding the publication of this paper.
Adetunji A.E., Sershen, Varghese B., and Pammenter N., 2021. Effects of exogenous application of five antioxidants on vigour, viability, oxidative metabolism and germination enzymes in aged cabbage and lettuce seeds. South African J. Bot., 137, 85-97,
AL-Aloosy Y.A.M., AL-Tameemi A.J.H., and Jumaa S.S., 2019. The role of enzymatic and non-enzymatic antioxidants in facing the environmental stresses on plant: A review. Plant Archives, 19(1), 1057-1060.
AOAC., 2010. Official methods of analysis of the Association of Official Analytical Chemists International, Arlington, VA, USA.
Avdeeva V., Zorina E., Bezgina J., and Kolosova O., 2018. Influence of ozone on germination and germinating energy of winter wheat seeds. Eng. Rur. Develop., 23, 543-546, https//
Bantacut T., 2017. Soybeans development for food, industrial and economy security. J. Pangan, 26, 81-96.
Baqasi L.A., Qari H.A., Al-Nahhas N., Badr R.H., Taia W.K., El-Dakak R.A., and Hassan I.A., 2018. Effects of low concentrations of ozone (O3) on metabolic and physiological attributes in wheat (Triticum Aestivum L.) plants. Biomed Pharmacol. J., 11(2), 929-934, https//
Baskakov I.V., Orobinsky V.I., Gulevsky V.A., Gievsky A.M., and Chernyshov A.V., 2020. Influence of ozonation in seed storage on corn grain yield and its quality. IOP Conf. Series: Earth Environ. Sci., 488, 012007, https//
Bhatia A., Mina U., Kumara V., Tomera R., Kumarc A., Chakrabartia B., Singh R.N., and Singh B., 2021. Effect of elevated ozone and carbon dioxide interaction on growth, yield, nutrient content and wilt diseasese verity in chickpea grown in Northern India. Heliyon, 7, e06049,
Çetinkaya N., Pazarlar S., and Paylan I.C., 2022. Ozone treatment inactivates common bacteria and fungi associated with selected crop seeds and ornamental bulbs. Saudi J. Biol. Sci., 29, 103480, https//
Cheng M.H. and Rosentrater K.A., 2017. Economic feasibility analysis of soybean oil production by hexane extraction. Ind. Crops Prod., 108, 775-785, https//
Dadlani M., Gupta A., Sinha S.N., and Kavali R., 2023. Seed Storage and Packaging. In: Seed Science and Technology (eds M. Dadlani, D.K. Yadava). Springer, Singapore, https//
Dong X., Sun L., Agarwal M., Maker G., Han Y., Yu X., and Ren Y., 2022. The effect of ozone treatment on metabolite profile of germinating barley. Foods, 11, 1211, https//
Ehrhardt-Brocardo N.C.M., Coelho C.M.M., and Souza C.A., 2022. Storage protein composition during germination and its association with physiological seed quality in common bean. Acta Sci. Agron., 44, e53434, https//
Finch-Savage W.E. and Bassel G.W., 2016. Seed vigour and crop establishment: extending performance beyond adaptation. J. Exp. Bot., 67(3), 567-591, https//
Isikber A.A. and Athanassiou C.G., 2015. The use of ozone gas for the control of insects and microorganisms in stored products. J Stored Prod. Res., 64, 139-145, https//
ISTA., 2015. International Rules for Seed Testing, Bassersdorf, Switzerland, International Seed Testing Association.
Jaques L.B.A., Coradi P.C., Rodrigues H.E., Dubal I.T.P., Padia C.L., Lima R.E., and Souza G.A.C.D., 2022. Post-harvesting of soybean seeds-engineering, processes technologies, and seed quality: a review. Int. Agrophys., 36, 59-81, https//
Kaur R., Chandra J., and Keshavkant S., 2021. Nanotechnology: an efficient approach for rejuvenation of aged seeds. Physiol. Mol. Biol. Plants, 27, 399-415, https//
Koskosidis A., Khah E.M., Pavli O.I., and Vlachostergios D.N., 2022. Effect of storage conditions on seed quality of soybean (Glycine max L.) germplasm. AIMS Agric. Food, 7(2), 387-402, https//
Lamarca E.V. and Barbedo C.J., 2012. Short storability of Caesalpinia echinata Lam. seeds as a consequence of oxidative processes. Hoehnea, 39, 577-586, https//
Li Y., Wang Y., Xue H., Pritchard H.W., and Xiaofeng W., 2017. Changes in the mitochondrial protein profile due to ROS eruption during ageing of elm (Ulmus pumila L.) seeds. Plant Physiol. Biochem., 114, 72-87, https//
Mohammadi F., Marti A., Nayebzadeh K., Hosseini S.M., and Tajdar-oranj B., 2021. Effect of washing, soaking and pH in combination with ultrasound on enzymatic rancidity, phytic acid, heavy metals and coliforms of rice bran. Food Chem., 334, 127583, https//
Mosneaga A., Nedeff V., Sandu I., Lozovanu P., Mosnegutu E., Lehadus M.P., and Sandu I.G., 2020. Utilization of ozone and composite materials in the seed treatment to stimulate the germination and growth of agricultural crops. Rev. Chim., 71(2), 365-370, https//
Nickhil C., Mohapatra D., Kar A., Giri S.K., Tripathi M.K., and Sharma Y., 2021. Gaseous ozone treatment of chickpea grains, part I: Effect on protein, amino acid, fatty acid, mineral content, and microstructure. Food Chem., 345, 128850, https//
Normov D., Chesniuk E., Shevchenko A., Normova T., Gagedman R., Pozhidaev D., Bohinc T., and Trdan S., 2019. Does ozone treatment of maize seeds influence their germination and growth energy? Acta Agric. Slovenica, 114(2), 251-258, https//
Obadi M., Zhu K.X., Peng W., Noman A., Mohammed K., and Zhou H.M., 2018. Characterization of oil extracted from whole grain flour treated with ozone gas. J. Cereal Sci., 79, 527-533, https//
Pandiselvam R., Thirupathi V., and Anandakumar S., 2015. Reaction kinetics of ozone gas in paddy grains. J. Food Proc. Engin., 38(6), 594-600, https//
Pandiselvam R., Mayookha V.P., Kothakota A., Sharmila L., Ramesh S.V., Bharathi C.P., Gomathy K., and Srikanth V., 2019. Impact of ozone treatment on seed germination - a systematic review. Ozone: Sci. Engin., 42(9), 331-346, https//
Rinaldi J., Arya N.N., Mahaputra I.K., Elisabeth D.A.A., Resiani N.M.D., Arsana I.G.K.D., and Silitonga T.F., 2023. Production factors, technical, and economic efficiency of soybean (Glycine max L. Merr.) farming in Indonesia. Open Agric., 8, 20220194, https//
Rodrigues V.O., Costa F.R., Nery M.C., Cruz S.M., de Melo S.G.F., and de Carvalho M.L.M., 2015. Treating sunflower seeds subjected to ozonization. J Seed Sci., 37, 202-210, https//
Sahab A.F., Hassanien F.R., El-Nemr S.E., Abdel-Alim H.A., and Abdel-Wahhab M.A., 2013. Effect of ozone gaseous on aflatoxin degradation and fat and protein content in peanut seeds. J. Appl. Sci. Res., 9, 2170-2175.
Sharaf-Eldin M.A., Alshallash K.S., Alharbi K.R., Alqahtani M.M., Etman A.A., Yassin A.M., Azab E.S., and El-Okkiah S.A.F., 2022. Influence of seed soaking and foliar application using ozonated water on two sweet pepper hybrids under caged stress. Sustainability, 14, 13453, https//
Shelar V.R., Shaikh R.S., and Nikam A.S., 2008. Soybean seed quality during storage: a review. Agric. Rev., 29(2), 125-131.
Shingala A.M. and Dabhi M.N., 2022. Influence of ozone treatment on wheat (Triticum aestivum) germination during bulk storage. J. Cereal Res., 14(3), 283-290, https//
Silva I.L., de Camargo F.R.T., de Souza R.T.G., Teixeira I.R., and Kikuti H., 2019. Storage of soybean seeds treated with chemicals. Semina: Ciencias Agrarias, 40(6), 2961-2972, https//
Sivaranjani S., Prasath V.A., Pandiselvam R., Kothakota A., and Khaneghah A.M., 2021. Recent advances in applications of ozone in the cereal industry. LWT - Food Sci. Technol., 146, 111412, https//
Szumigaj-Tarnowska J., Szafranek P., Uliński Z., and Ślusarski C., 2020. Efficiency of gaseous ozone in disinfection of mushroom growing rooms. J. Hort. Res., 28(2), 91-100, https//
Timotiwu P.B., Nurmauli N., and Khairunnisa F.H., 2022. Study of viability and vigor of new and aged soybean seeds. J. Agrotek Tropika, 10(4), 541-545, https//
Toci A.T., Neto V.J.M.F., Torres A.G., and Farah A., 2013. Changes in triacylglycerols and free fatty acids composition during storage of roasted coffee. LWT – Food Sci. Technol., 50, 581-590,
Uzun H., Ibanoglu E., Catal H., and Ibanoglu S., 2012. Effects of ozone on functional properties of proteins. Food Chem., 134, 647-654, https//
Vijayakumar H.P. and Vijayakumar A., 2015. Standardization of accelerated ageing duration to evaluate seed storability of soybean cultivars. Int. J. Agric. Sci. Res. (IJASR), 5, 93-98.
White S.D., Murphy P.T., Leandro L.F., Bern C.J., Beattie S.E., and van Leeuwen J., 2013. Mycoflora of high-moisture maize treated with ozone. J. Stored Prod. Res., 55, 84-89, https//
Xin X., Tian Q., Yin G., Chen X.L., Zhang J., Ng S., and Lu X., 2014. Reduced mitochondrial and ascorbate-glutathione activity after artificial ageing in soybean seed. J. Plant Physiol., 171, 140-147, https//
Zhang K., Zhang Y., Sun J., Meng J., and Tao J., 2021. Deterioration of orthodox seeds during ageing: Influencing factors, physiological alterations, and the role of reactive oxygen species. Plant Physiol. Biochem., 158, 475-485, https//
Journals System - logo
Scroll to top