Field effectiveness of gaseous ozone storage in alleviating damage to aged soybean seeds
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Research Center for Food Technology and Processing, National Research and Innovation Agency, Jl. Yogya-Wonosari KM 31.5, Gading, Playen Gunung Kidul, Yogyakarta 55861, Indonesia
Research Center for Horticultural and Estate Crops, National Research and Innovation Agency, Jl. Raya Bogor-Jakarta, Cibinong, Bogor 16911, Indonesia
Research Center for Food Crops, National Research and Innovation Agency, Jl. Raya Bogor-Jakarta, Cibinong, Bogor 16911, Indonesia
Research Center for Behavioral and Circular Economics, National Research and Innovation Agency, Jl. Jend. Gatot Subroto No.10, Jakarta 12710, Indonesia
Research Center for Sustainable Production System and Life Cycle Assessment, National Research and Innovation Agency, Gedung Pusat Inovasi dan Bisnis Teknologi No.720, Kawasan Puspitek Serpong, Tangerang Selatan 15314, Indonesia
Physics Department, Faculty of Science and Mathematics, Diponegoro University, Jl. Prof. Soedarto, Tembalang, Semarang 50275, Indonesia
Final revision date: 2023-09-21
Acceptance date: 2023-10-03
Publication date: 2023-10-31
Corresponding author
Indrie Ambarsari   

Research Center for Food Technology and Processing, National Research and Innovation Agency, Indonesia
Int. Agrophys. 2023, 37(4): 435–441
  • Ozone effectively extends the longevity of aged soybean seeds up to five months by increasing their viability;
  • Ozone triggers the antioxidant defence system and healing mechanism in aged soybean seeds;
  • The aged seed viability increased in ozone storage contributed to reducing seed losses and financial losses.
Ozonation is a promising method for maintaining seed quality. However, the ability of ozone to preserve the quality of aged seeds, which have already deteriorated, is still open to question. This study evaluated the effectiveness of ozone in alleviating ageing soybean seed damage and also the economic feasibility of ozone technology under field conditions. Samples under ozonation storage (they were stored in open containers and woven sacks) were compared with those stored under conventional storage conditions (they were stored in woven bags without ozonation). Ozonated samples were exposed to 150 g h–1 of ozone gas for three hours a day for a six month storage period. All samples (with and without ozonation) were stored at 18±5°C and 50±5% relative humidity. Observations were conducted every month with regard to the seeds germination percentage, vigour index, moisture content, protein and free fatty acids. The results showed that ozonation has the potential to increase the viability of aged soybean seeds for up to 5 months. However, the decrease in the protein and free fatty acids levels in the ozonated seeds occurred more rapidly than in the control. Moreover, no significant difference was found between the ozonated seeds in open containers and sacks during storage, except for their moisture content. Ozone application on aged soybean seeds was found to be financially feasible with an ratio of revenue to cost value of 1.73, an return on investment of 74.63% and a payback period of 1.34 years.
The authors declare that there is no conflict of interest regarding the publication of this paper.
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