Soil microbial and nutrient dynamics influenced by irrigation-induced salinity and sewage sludge incorporation in sandy – loam textured soil
Ankush Ankush 1  
,   Ram Prakash 1  
,   Rakesh Kumar 2  
,   Vikram Singh 3  
,   Harender Harender 3  
,   Vijay Kant Singh 4  
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Department of Soil Science, CCS HAU, Haryana - 125 001, India
Department of Microbiology, CCS HAU, Haryana - 125 001, India
Department of Agronomy, CCS HAU, Haryana - 125 001, India
Department of Natural Resource Management, Birsa Agricultural University, Ranchi, Jharkhand - 834006, India
Ankush Ankush   

Department of Soil Science, CCS HAU, Haryana - 125 001, India
Final revision date: 2020-10-19
Acceptance date: 2020-10-23
Publication date: 2020-11-13
Int. Agrophys. 2020, 34(4): 451–462
The use of sewage sludge in agriculture is known to be environmentally friendly as it is a practical way of recycling municipal waste. A short-term experiment was carried out to study the changes in soil chemical and microbiological properties influenced through the addition of sewage sludge along with saline irrigation under a pearl millet-wheat crop rotation. There were three irrigation (canal water (0.35 dS m-1), 8 and 10 dS m-1 electrical conductivity saline water) and five fertilizer treatments (control-F1, sewage sludge (5 t ha-1)-F2, sewage sludge (5 t ha-1) +50% recommended dose of fertilizer (RDF)-F3, sewage sludge (5 t ha-1)+75% RDF-F4 and RDF-F5). The results showed that soil organic carbon, and available nitrogen, and phosphorus were reduced significantly under saline conditions but there was an increase in available potassium with the increasing salinity levels of the irrigation water. A significant reduction in soil microbial biomass carbon and enzyme activities was caused by 8 and 10 dS m-1 as compared to 0.35 dS m-1 at different growth stages of crops. Treatment F4 attained the highest soil microbial activity at each crop growth stage by a significant margin among all of the fertilizer treatments, which is associated with a substantial build-up of organic carbon and available NPK in the soil. Soil microbial activities followed a particular trend: at 35 and 75 days after sowing>harvest>sowing in pearl millet and wheat, respectively. Sewage sludge dumping is a major problem in India, and the present study suggests that by applying sewage sludge, soil biological health is improved; therefore its use in agriculture is recommended to farmers.
Ahmed H.K., Fawy H.A., and Abdel-Hady E.S., 2010. Study of sewage sludge use in agriculture and its effect on plant and soil. Agriculture and Biology J. North America, 1(5), 1044-1049.
Akmal M., Altaf M.S., Hayat R., Hassan F.U., and Islam M., 2012. Temporal changes in soil urease, alkaline phosphatase and dehydrogenase activity in rainfed wheat field of Pakistan. J. Animal Plant Sci., 22(2), 457-462.
Angin I., Aslantas R., Gunes A., Kose M., and Ozkan G., 2016. Effects of sewage sludge amendment on some soil properties, growth, yield and nutrient content of raspberry (Rubus idaeus L.). Erwerbs-obstbau, 59(2), 93-99.
Antil R.S., Singh A., and Dahiya S.S., 2002. Practical Mannual for Soil and Plant Analysis. Department of Soil Science, CCS Haryana Agricultural University, Hisar - 125004.
Aranyos J.T., Tomocsik A., Makádi M., Mészáros J., and Blasko L., 2016. Changes in physical properties of sandy soil after long-term compost treatment. Int. Agrophys., 30(3), 269-274.
Awad A.S., Edwards D.G., and Campell L.C., 1990. Phosphorus enhancement of salt tolerance of tomato. Crop Science, 30, 123-128.
Bhat M.A., Grewal M.S., Dinesh, Singh I., and Grewal K.S., 2017. Geoinformatics for quantifying salt affected soils in Gohana, Haryana using soil techniques. Int. J. Current Microbiol. Appl. Sci., 6(9), 835-858.
Borase D.N., Nath C.P., Hazra K.K., Senthilkumar M., Singh S.S., Praharaj C.S., Singh U., and Kumar N., 2020. Long-term impact of diversified crop rotations and nutrient management practices on soil microbial functions and soil enzymes activity. Ecological Indicators, 114, 106322.
Bourioug M., Mazzitelli J.Y., Marty P., Budzinski H., Aleya L., Bonnafe E., and Geret F., 2017. Assessment of Lemna minor (duckweed) and Corbicula fluminea (freshwater clam) as potential indicators of contaminated aquatic ecosystems: responses to presence of psychoactive drug mixtures. Environ. Sci. Pollut. Res., s11356-017-8447-1.
Chesnin L. and Yien C.H., 1950. Turbidimetric determination of available sulphates. Soil Sci. Soc. Am. J., 15, 149-151.
CPCB, 2006. Status of sewage treatment in India November 2005 status report CUPS/61/2005-06.
Curci M., Lavecchia A., Cucci G., Lacolla G., De Corato U., and Crecchio C., 2020. Short-term effects of sewage sludge compost amendment on semi-arid soil. Soil Systems, 4(3), 48.
Datta K.K. and De Jong C., 2002. Adverse effect of waterlogging and soil salinity on crop and land productivity in northwest region of Haryana, India. Agricultural Water Manag., 57(3), 223-238.
Dotaniya M.L., Kushwah S.K., Rajendiran S., Coumar M.V., Kundu S., and Rao A.S., 2014. Rhizosphere effect of kharif crops on phosphatases and dehydrogenase activities in a Typic Haplustert. National Academy Science Letters, 37(2), 103-106.
Egamberdieva D., Renella G., Wirth S., and Islam R., 2010. Secondary salinity effects on soil microbial biomass. Biology Fertility Soils, 46, 445-449.
Eid E.M., Alrumman S.A., El-Bebany A.F., Fawy K.F., Taher M.A., Hesham A.E.L., El-Shaboury G.A., and Ahmed M.T., 2019. Evaluation of the potential of sewage sludge as a valuable fertilizer for wheat crop. Environ. Sci. Poll. Res., 26(1), 392-401.
Ghollarata M. and Raiesi F., 2007. The adverse effects of soil salinization on the growth of Trifolium alexandrinum L. and associated microbial and biochemical properties in a soil from Iran. Soil Biol. Biochem., 39, 1699-1702.
Hamuda H.B. and Ligetvari F., 2011. Impacts of municipal sewage sludge on dynamics of organic matter and biological activities in clay loam brown forest soil. J. Residuals Sci. Technol., 8(4), 143-149.
Huang W., Liu J., Zhou G., Zhang D., and Deng Q., 2011. Effects of precipitation on soil acid phosphatase activity in three succession forests in Southern China. Biogeosciences, 8, 1901-1910.
Iwai C.B., Oo A.N., and Topark-ngarm B., 2012. Soil property and microbial activity in natural salt affected soils in an alternating wet-dry tropical climate. Geoderma, 189, 144-152.
Islam N.F. and Borthakur 2016. Effect of different growth stages on rice crop on soil microbial and enzyme activities. Tropical Plant Research, 3 (1), 40-47.
Jezierska-Tys S. and Frąc M., 2009. Impact of dairy sewage sludge on enzymatic activity and inorganic nitrogen concentrations in the soils. Int. Agrophys., 23(1), 31-37.
Jingjing S., Jiaheng M., Xiaoying W., Cheng W., and Jun Y., 2015. Microorganism quality and enzyme activities in wheat field subjected to different nitrogen fertilizer rates. Open Biotechnol. J., 9, 204-208.
Kumar V., Kumar D., and Kumar P., 2007. Declining water table scenario in Haryana – A review. Water and Energy International, 64(2), 32-34.
Kumawat R.M., Pathan A.R.K., and Yadav K.K., 2011. Effect of FYM and phosphorus on soil properties and nutrient content of fenugreek under saline water irrigation. Ecology, Environment and Conservation, 17(4), 711-716.
Leogrande R. and Vitti C., 2018. Use of organic amendments to reclaim saline and sodic soils: a review. Arid Land Research and Management, 33(1), 1-21.
Maiti R.K. and Bidinger F.R., 1981. Growth and development of the pearl millet plant Research Bulletin No 6. Patancheru, A.P., India, International Crops Research Institute for the Semi-Arid Tropics.
Maurya B.R., Singh V., and Dhyani P.P., 2011. Enzymatic activities and microbial population in agric-soils of Almora District of Central Himalaya as influenced by altitudes. Int. J. Soil Sci., 6, 238-248.
McGrath S.P., Zhao F.J., Dunham S.J., Crosland A.R., and Coleman K., 2000. Long term changes in extractability and bioavailability of zinc and cadmium after sludge application. J. Environmental Quality, 29, 875-883.
Medina J., Monreal C., Barea J.M., Arriagada C., Borie F., and Cornejo P., 2015. Crop residue stabilization and application to agricultural and degraded soils: a review. Waste Management, 42, 41-54.
Meena M.D., Joshi P.K., Jat H.S., Chinchmalatpure A.R., Narjary B., Sheoran P., and Sharma D.K., 2016. Changes in biological and chemical properties of saline soil amended with municipal solid waste compost and chemical fertilizers in a mustard-pearl millet cropping system. Catena, 140, 1-8.
Meena M.D., Narjary B., Sheoran P., Jat H.S., Joshi P.K., Chinchmalatpure A.R., Yadav G., Yadav R.K., and Meena M.K., 2018. Changes of phosphorus fractions in saline soil amended with municipal solid waste compost and mineral fertilizer in a mustard-pearl millet cropping system. Catena, 160, 32-40.
Melo W.J., Aguiar P.S., de Melo G.M., Peruca M., and Valeria P., 2007. Nickel in a tropical soil treated with sewage sludge and cropped with maize in a long-term field study. Soil Biol. Biochemistry, 39, 1341-1347.
Muscolo A., Mallamaci C., Panuccio M.R., Caputo R., and De Pascale S., 2011. Effect of long-term irrigation water salinity on soil properties and microbial biomass. Ecological Questions, 14, 77-79.
Nannipieri P., 1994. The potential use of soil enzymes as indicators of productivity, sustainability and pollution. pp. 238-244. In: Soil Biota Management in Sustainable Farming Systems (Eds C.E. Pankhurst, B.M. Double, V.V.S.R. Gupta, and P.R. Grace). CSIRO, East Melbourne.
Nannipieri P., Giagnoni L., Renella G., Puglisi E., Ceccanti B., Masciandaro G., Fornasier F., Moscatelli M.C., and Marinari S., 2012. Soil enzymology: classical and molecular approaches. Biol. Fertility Soils, 48, 743-762.
Nihorimbere V., Ongena M., Smargiassi M., and Thonart P., 2011. Beneficial effect of the rhizosphere microbial community for plant growth and health. Biotechnology, Agronomy, Soci. Environ., 15(2), 327-337.
Page A.L., Miller R.H., and Keeney D.R., 1982. Methods of Soil Analysis. 2nd Edn., American Society of Agronomy, Madison, USA.
Pascual I., Antolin M.C., Garcia C., Polo A., and Sanchez-Diaz M., 2007. Effect of water deficit on microbial characteristics in soil amended with sewage sludge or inorganic fertilizer under laboratory conditions. Bioresour. Technol., 98, 29-37.
Patel A. and Patra D.D., 2014. Influence of heavy metal rich tannery sludge on soil enzymes vis-a-vis growth of Tagetes minuta, an essential oil bearing crop, Chemosphere, 112, 323-332.
Rakshit R., Patra A.K., Pal D., Kumar M., and Singh R., 2012. Effect of elevated CO2 and temperature on nitrogen dynamics and microbial activity during wheat (Triticum aestivum L.) growth on a subtropical inceptisol in India. J. Agronomy Crop Sci., 198(6), 452-465.
Richard L.A., 1954. Diagnosis and improvement of saline and alkaline soils. USDA Handbook Number 60, Washington D.C. Estimation of available phosphorous in soil by extraction with sodium bicarbonate. Circulars USDA. 939.
Rietz D.N. and Haynes R.J., 2003. Effect of irrigation-induced salinity and sodicity on soil microbial activity. Soil Biology Biochemistry, 35, 845-854.
Sardans J. and Penuelas J., 2005. Drought decreases soil enzyme activity in a Mediterranean Quercus ilex L. forest. Soil Biology Biochemistry, 37, 455-461.
Sardans J., Penuelas J., and Estiarte M., 2008. Changes in soil enzymes related to C and N cycle and in soil C and N content under prolonged warming and drought in a Mediterranean shrub land. Appl. Soil Ecol., 39, 223-235.
Sardinha M., Müller T., Schmeisky H., and Joergensen R.G., 2003. Microbial performance in soils along a salinity gradient under acidic conditions. Appl. Soil Ecol., 23, 237-244.
Sekhon K.S., Kaur A., Thaman S., Sidhu A.S., Garg N., Choudhary O.P., Buttar G.S., and Chawla N., 2019. Irrigation water quality and mulching effects on tuber yield and soil properties in potato (Solanum tuberosum L.) under semi-arid conditions of Indian Punjab. Field Crops Res.,
Shah S.A. and Shah Z., 2011. Changes in soil microbial characteristics with elevated salinity. Sarhad J. Agric., 27(2), 233-244.
Sharma S. and Dhaliwal S.S., 2019. Effect of sewage sludge and rice straw compost on yield, micronutrient availability and soil quality under rice-wheat system. Communications in Soil Science and Plant Analysis,
Sheoran O.P., Tonk D.S., Kaushik L.S., Hasija R.C., and Pannu R.S., 1998. Statistical Software Package for Agricultural Research Workers. Recent Advances in information theory, Statistics & Computer Applications by Hooda, D.S., Hasija, R.C., Department of Mathematics Statistics, CCS HAU, Hisar (139-143).
Siddikee M.A., Tipayno S.C., Kim K., Chung J.B., and Sa T., 2011. Influence of varying degree of salinity-sodicity stress on enzyme activities and bacterial populations of coastal soils of Yellow Sea, South Korea. J. Microbiol. Biotechnol., 2, 341-346.
Singh P., Choudhary O.P., and Mavi M.S., 2018a. Irrigation-induced salinization effects on soil chemical and biological properties under cotton-wheat rotation in loamy sand soil in Northwest India. J. Indian Soc. Soil Sci., 66(4), 386-391.
Singh S.K., Kumar M., Singh R.P., Bohra J.S., Srivastva J.P., Singh S.P., and Singh Y.V., 2018b. Conjoint application of organic and inorganic sources of nutrients on yield, nutrient uptake and soil fertility under rice-wheat system. J. Indian Soc. Soil Sci., 66(3), 287-294.
Singh R.P. and Agrawal M., 2010. Variations in heavy metal accumulation, growth and yield of rice plants grown at different sewage sludge amendment rates. Ecotoxicology and Environment Safety, 73(4), 632-641.
Singh K., 2016. Microbial and enzyme activities of saline and sodic soils. Land Degradation and Development, 27(3), 706-718.
Skowrońska M., Bielińska E.J., Szymański K., Futa B., Antonkiewicz J., and Kołodziej B., 2020. An integrated assessment of the long-term impact of municipal sewage sludge on the chemical and biological properties of soil. Catena, 189, 104484.
Srinivasarao C., Kundu S., Grover M., Manjunath M., Sudhanshu S.K., Patel J.J., Singh S.R., Singh R.P., Patel M.M., Arunachalam A., and Soam S.K., 2018. Effect of long term application of organic and inorganic fertilizers on soil microbial activities in semi-arid and sub-humid rainfed agricultural systems. Tropical Ecology, 59(1).
Tabatabai M.A. and Bremner J.M., 1972. Assay of urease activity in soils. Soil Biology Biochemistry, 4, 479-487.
Tejada M., Garcia C., Gonzalez J.L., and Hernandez M.T., 2006. Organic amendment based on fresh and composted beet vinasse. Soil Sci. Soc. America J., 70, 900-908.
Todd D.K., 1980. Groundwater Hydrology, Second edition, John Wiley and Sons, New York.
Usman A.R.A., 2015. Influence of NaCl induced salinity and Cd toxicity on respiration activity and Cd availability to barley plants in FYM-amended soil. Appl. Environ. Soil Sci.,
Vance E.D., Broolers P.C., and Jenkinson D.S., 1987. An extraction method for measuring soil microbial biomass carbon. Soil Biology Biochem., 19, 703-707.
Wang J., Wang H., Cao Y., Bai Z., and Qin Q., 2016. Effects of soil and topographic factors on vegetation restoration in opencast coal mine dumps located in a loess area. Scientific Reports, 6(1), 1-11.
Wang Q.K., Wang S.L., and Liu Y.X., 2008. Responses to N and P fertilization in a young Eucalyptus dunnii plantation: Microbial properties, enzyme activities and dissolved organic matter. Applied Soil Ecology, 40, 484-90.
Yadav S.S., Tikkoo A., and Singh J.P., 2012. Effect of potassium on pearl millet-wheat Cropping system in coarse textured soils of Southern Haryana. J. Indian Soc. Soil Sci., 60(2), 145-149.
Yao S., Qin J., Peng X., and Zhang B., 2009. The effect of vegetation on restoration of physical stability of severely degraded soil in China. Ecological Engineering, 35, 723-734.
Yuan B., Li Z., Liu H., Gao M., and Zhang Y., 2007. Microbial biomass and activity in salt affected soils under arid conditions. Applied Soil Ecology, 35, 319-328.
Zadoks J.C., Chang T.T., and Konzak C.F., 1974. A decimal code for the growth stages of cereals. Weed Res., 14, 415-421.
Zahir Z.A., Malik M.A.R., and Arshad M., 2001. Soil enzymes research: a review. Online J. Biol. Sci., 1, 299-307.